中国畜牧兽医2016，Vol.43Issue(9)：2265-2271,7.DOI:10.16431/j.cnki.1671-7236.2016.09.007
牦牛 HOXA1基因的时空表达及其对机体调控机制的研究
Expression Pattern of HOXA1 Gene and Its Putative Regulating Mechanism of Yak
摘要
Abstract
The aim of study was to obtain the yak HOXA1 gene sequence and related bioinformat-ics information,and analyze its expression spectrum and temporal expression profiles for providing related information of the formation of multiple vertebrae and its mechanism.RT-PCR technology was applied to clone the yak HOXA1 gene,semi-quantitative RT-PCR technique was used to de-tect the gene expression level in multiple organisms,and Real-time quantitative PCR technique was used to detect the gene expression level in the organisms in different development periods. The results showed that the obtained HOXA1 gene was 885 bp including the ORF of 870 bp,en-coding 290 amino acids.The yak HOXA1 gene shared a homology of 75.4% to 98.1% with ordi-nary cow,wild yak,human,wild boar,horse,mice,chimpanzees,jungle fowl and wild boar.There were different expression level of HOXA1 gene in heart,liver,spleen,lung,kidney,large intes-tine,small intestine,muscle,stomach,ovaries,uterus,fallopian tubes,breast and testicular tissue. With the growth of the yak,HOXA1 gene expression level in tissue also increased.关键词
牦牛/HOXA1 基因/克隆/序列分析/组织表达/时序表达Key words
yak/HOXA1 gene/cloning/sequence analysis/tissue expression/temporal expression分类
生物科学引用本文复制引用
张亚南,石仙,熊显荣,兰道亮,李键..牦牛 HOXA1基因的时空表达及其对机体调控机制的研究[J].中国畜牧兽医,2016,43(9):2265-2271,7.基金项目
国家科技支撑计划(2012BAD13B06) （2012BAD13B06）
国家公益性行业(农业)科研专项(201203009) （农业）
西南民族大学研究生创新性项目(CX2015SZ068) （CX2015SZ068）
