中国畜牧兽医2016，Vol.43Issue(9)：2272-2278,7.DOI:10.16431/j.cnki.1671-7236.2016.09.008
马鹿 Zfx/Zfy 基因的克隆及序列分析
Cloning and Sequence Analysis of Zfx/Zfy Gene in Cervus elaphus
摘要
Abstract
Specific primers were designed according to the mRNA sequence of Bos taurus Zfx/Zfy genes provided in GenBank.Zfx/Zfy sequences of Cervus elaphus were cloned and ana-lyzed.The results showed that the cloned Zfx gene sequence of Cervus elaphus was 2 1 15 bp (GenBank accession No:KP257294.1 )that encoded 696 amino acids,the cloned Zfy gene se-quence was 2 406 bp (GenBank accession No:KU041539)that encoded 801 amino acids.The phy-logenetic trees of Zfx and Zfy amino acid were obtained using the Neighbor-Joining method of DNAStar software.Analysis showed that Zfx of Cervus elaphus was closely related to Homo sa-piens ,compared with Bison,Pantholops hodgosoniiand ,Panthera tigris ,Felis catus ,Bos taurus , Canis lupus familiaris ,Ovis aries .Zfy of Cervus elaphus was closely related to Bos taurus ,Bi-son,Ovis aries ,Pantholops hodgosonii compared with others.Zfx/Zfy genes of Cervus elaphus were cloned and sequenced for the first time,and this research provided a foundation for further studying the structure and biological functions of the proteins.关键词
马鹿/Zfx/Zfy 基因/克隆/序列分析Key words
Cervus elaphus/Zfx/Zfy gene/clone/sequence analysis分类
生物科学引用本文复制引用
魏丽敏,孙丽荣,张永生,席继锋,李超程,王绪海,王香祖,贾斌..马鹿 Zfx/Zfy 基因的克隆及序列分析[J].中国畜牧兽医,2016,43(9):2272-2278,7.基金项目
新疆生产建设兵团动物疾病防控重点实验室开放基金(BTDJ04) （BTDJ04）
