中国畜牧兽医2016，Vol.43Issue(9)：2366-2372,7.DOI:10.16431/j.cnki.1671-7236.2016.09.022
PCV2通过 NF-κB/NLRP3信号通路调控体外培养 PAMs 分泌 IL-1β
PCV2 Regulates PAMs Secreting IL-1βthrough the NF-κB/NLRP3 Signalling Pathway in vitro
摘要
Abstract
This study investigated the molecular mechanism of interleukin-1β (IL-1β)production in porcine alveolar macrophages (PAMs),which were infected by porcine circovirus 2 (PCV2). Two 6-week-old piglets were selected as experiment animals,which were free of PCV2 and por-cine reproductive and respiratory syndrome virus (PRRSV)antibody and antigen.The alveolar macrophages were isolated aseptically and cultured in vitro as this study object.IL-1βin the super-natants from PCV2-infected PAMs was assayed by ELISA.The expressions of NLRP3 and ASC in the PCV2-infected PAMs were detected by Real-time quantitative PCR.The regulatory function of NLRP3 and NF-κB in IL-1βsecretion by the PCV2-infected PAMs was analyzed by small inter-fering RNA (siRNA)and NF-κB inhibitor BAY1 1-7082,respectively.The results showed that the expressions of IL-1β,NLRP3 (except for 1 h)and ASC (except for 1 and 3 h)in cell culture su-pernatants or PCV2-infected PAMs were significantly increased (P < 0.05;P < 0.01 ).siRNA could lead to 58.3% of NLRP 3 gene silencing.IL-1βsecretion in the PCV2-infected PAMs,which were companied by NLRP 3 gene silencing,was significantly decreased (P <0.05).The IL-1βlev-el in the PCV2-infected PAMs,which were treated with inhibitor BAY1 1-7082,was also signifi-cantly decreased.These results suggested that PCV2 regulated PAMs secreting IL-1βthrough the NF-κB/NLRP3 signal pathway in vitro .关键词
猪圆环病毒 2 型/猪肺泡巨噬细胞/白细胞介素-1β/核因子-kappa B/NLRP3Key words
PCV2/PAMs/IL-1β/NF-κB/NLRP3分类
生物科学引用本文复制引用
李海花,张蕾,杨春蕾,赵向华,乔家运,王文杰..PCV2通过 NF-κB/NLRP3信号通路调控体外培养 PAMs 分泌 IL-1β[J].中国畜牧兽医,2016,43(9):2366-2372,7.基金项目
国家自然科学基金项目(31402087) （31402087）
天津市农业科学院院长基金(13004) （13004）
