中国畜牧兽医2016，Vol.43Issue(9)：2401-2411,11.DOI:10.16431/j.cnki.1671-7236.2016.09.027
细毛羊 LAMB 1基因外显子多态性及其与羊毛纤维直径的关联性分析
Polymorphism of LAMB 1 Gene and Its Association Analysis with the Fiber Diameter in Fine-wool Sheep
摘要
Abstract
This study aimed to research the single nucleotide polymorphism (SNPs)of LAMB 1 gene exon and its correlation with the fiber diameter in Fine-wool sheep.Based on DNA pools with the Re-sequencing technology to gain SNPs data,totally screened 20 SNPs of LAMB 1 gene exon regions,at the same time,combining with direct sequencing method,PCR-SSCP and bioin-formatics software to verify the accuracy of 10 missense mutations.The genetic effects of LAMB1 on fiber diameter at Xinjiang Kunes farm were analyzed by the GLM of SAS,totally 300 sheep. The results showed that 20 SNPs of LAMB 1 gene were screened,which included 10 synonymous mutation SNPs and 10 non-synonymous mutation SNPs,after the validation,there were 7 mis-sense mutation SNPs which led to the nature of protein change,3 SNPs were not mutated. LAMB1 protein became increasingly hydrophobic after mutating,predicting that was insoluble protein.TT genotype in the fiber diameter of the individual value was significantly higher than TC and CC genotypes on SNP5(P <0.05),GG and TT genotypes in the fiber diameter of the individ-ual value was significantly higher than GT genotype on SNP9 (P <0.05 ).Although,all of the SNPs were completely detected by DNA pool with the Re-sequencing technology and minimized the false positives,it still need to be validated by direct sequencing.The results revealed that LAMB 1 gene existed highly genetic diversity,mutated SNPs were unevenly distributed in exons, SNP5(rs159769941)and SNP9(rs159769901)could be considered as an effective genetic markers of Fine-wool sheep on fiber diameter.关键词
DNA 混池/重测序/LAMB 1 基因/SNPsKey words
DNA pool/Re-sequencing/LAMB 1 gene/SNPs分类
农业科技引用本文复制引用
赵冰茹,黄锡霞,田可川,付雪峰,徐新明,柏妍,田月珍..细毛羊 LAMB 1基因外显子多态性及其与羊毛纤维直径的关联性分析[J].中国畜牧兽医,2016,43(9):2401-2411,11.基金项目
国家自然科学基金(31360543、31460593) （31360543、31460593）
农业部体系项目(CARS-40) （CARS-40）
新疆农业大学产学研联合培养研究生示范基地项目(xjaucxy-yjs-20152012) （xjaucxy-yjs-20152012）
