中国畜牧兽医2016，Vol.43Issue(9)：2412-2417,6.DOI:10.16431/j.cnki.1671-7236.2016.09.028
水牛 GABA A 型受体π亚基基因的克隆及其在乳腺组织中的表达
Cloning of GABRP Gene and Its Expression in Buffalo Mammary Tissue
摘要
Abstract
GABA is an important inhibitory neurotransmitter in the brain,beside the function of transmissing information,which plays an important role in endocrine tissues.In this study,the CDS of buffalo GABRP gene was cloned and its biological information was analyzed,the expres-sion of GABRP in buffalo mammary tissue was also examined by Real-time quantitative PCR and immunohistochemistry.The RT-PCR results showed that GABRP CDS fragment was obtained successfully from Guangxi local buffalo,which shared 99% with that of river buffalo,its length was 1 401 bp.The phylogenetic tree showed that GABRP had high conservation in different spe-cies.The immunohistochemistry results showed that buffalo GABRP protein located at the alveo-lar epithelial cells of buffalo mammary.The expression of GABRP gene in non-lactation buffalo mammary was significantly higher than that of lactation tissue (P <0.05).The results laid a foun-dation for further study the function of GABRP gene in the development of buffalo mammary gland.关键词
水牛/GABRP 基因/乳腺组织Key words
buffalo/GABRP gene/mammary tissue分类
生物科学引用本文复制引用
郝文艳,陈培芳,代小丽,王萍,吴大平,石德顺,李湘萍..水牛 GABA A 型受体π亚基基因的克隆及其在乳腺组织中的表达[J].中国畜牧兽医,2016,43(9):2412-2417,6.基金项目
国家自然科学基金(31560632) （31560632）
广西自然科学基金项目(2012GXNSFCB053002、2014GXNSFAA118084) （2012GXNSFCB053002、2014GXNSFAA118084）
国家留学回国基金项目(BLH120499) （BLH120499）
