中国畜牧兽医2016，Vol.43Issue(9)：2461-2467,7.DOI:10.16431/j.cnki.1671-7236.2016.09.035
伪狂犬病病毒变异株的鉴定及伪狂犬病的防控
Identification of Pseudorabies Virus Variant and Control of Pseudorabies
摘要
Abstract
Due to variant strain,pseudorabies virus (PRV)has broken out again and spread in China since 201 1.A swine farm in Guangdong province was found pseudorabies (PR)symptoms-like miscarriage after introduction.The study was carried out to identify and control the PR.Ser-um of sows with and without miscarriage were randomly collected and the PRV gB and gE were detected by ELISA method,and brain tissues of sick piglets were sampled and the PRV g H gene was tested by PCR.All the sows in the farm were emergently inoculated PRV variant strainin ac-tivated vaccine.Serum before and after immunization were collected and detected by ELISA and micro-serum neutralization test.ELISA results showed that gE antibody of all the breeding sows with miscarriage were positive,and that of sows without miscarriage showed weekly positive;The average gB ELISA S/P value of sows with miscarriage was as high as 4.0 ,while that of sows without miscarriage was over 3.0.PCR of 3 sick piglets were all positive and the sequence of gB gene was 100% identical to BJ-YT-2012,a wide variant stain in 2012.The result of detection of the sows serum at before and after immunization showed that the S/P value of gB rose up from 1.603 before immunization to 2.88 at four weeks after immunization,and the neutralizing antibod-y rose up from 1∶24 to 1∶213.This agreed with the results that the sows showed less probabili-ty of miscarriage since the first week after immunization and almost no miscarriage after two weeks after immunization.This study suggested that classical PRV vaccine was not effective in this case,while the vaccine made from the variant PRV strain was.关键词
伪狂犬病病毒/变异株/检测/鉴定/免疫Key words
pseudorabies virus/variant/detection/identification/immunization分类
农业科技引用本文复制引用
黄元,陈晶,赵翠玲,陈锦良,向蓉,王晓虎,向华,黄忠..伪狂犬病病毒变异株的鉴定及伪狂犬病的防控[J].中国畜牧兽医,2016,43(9):2461-2467,7.基金项目
广东省科技计划项目(2015A020224026、2015A020208007) （2015A020224026、2015A020208007）
广东省农业科学院院长基金项目(201628) （201628）
