中国畜牧兽医2016，Vol.43Issue(9)：2502-2507,6.DOI:10.16431/j.cnki.1671-7236.2016.09.041
猪精子质量的流式细胞检测分析
Detection and Analysis of the Pig Sperm Quality by Flow Cytometry
摘要
Abstract
The flow cytometry was used in this trail to determine the pig sperm quality,in order to ensure the sperm had complete functions during the fertilization process.Four stain methods of SYBR-14/PI (Propidium Iodide),YO-PRO-1/PI,PNA/PI,Mito tracker/YO-PRO-1 were used to detect the plasma membrane integrity,apoptosis,top body ntegrity and mitochondrial function of Rongchang pig sperm.SYBR-14 +/PI- expressed live sperm with complete plasma membrane, YO-PRO-1 -/PI- expressed live sperm,PNA-/PI- expressed live sperm had no top body reac-tion,and Mito Tracker+/YO-PRO-1 - expressed live sperm with high mitochondrial activity.The functional integrity of the tested sperm was evaluated according to the above comprehensive re-sults.Through the study of Rongchang pig sperm quality detection by flow cytometry,it sugges-ted that the method could provide theoretical basis for clinical diagnosis,and was very important for swine infertility treatments.关键词
荣昌猪/精子/流式细胞术/检测Key words
Rongchang pig/sperm/flow cytometry/detection分类
农业科技引用本文复制引用
吴梦,丁玉春,刘作华,刘文,罗林,葛良鹏..猪精子质量的流式细胞检测分析[J].中国畜牧兽医,2016,43(9):2502-2507,6.基金项目
863项目(2014AA021602) （2014AA021602）
重庆市国际合作项目(CSTC2013gjhz80002) （CSTC2013gjhz80002）
重庆市基础与前沿研究重大项目(cstc2013jcyjC80001) （cstc2013jcyjC80001）
重庆市农发资金项目(12402) （12402）
重庆市基本科研业务费项目(15447) （15447）
