中国药物评价2016，Vol.33Issue(3)：133-135,3.
HPLC法测定盐酸羟甲唑啉滴鼻液中苯扎溴铵的含量
Determination of Benzalkonium Bromide in Oxymetazoline Hydrochloride Nasal Drops by HPLC
赵晓頔 1张肖宁 1杨利红1
作者信息
- 1. 黑龙江省食品药品检验检测所，黑龙江哈尔滨150088
- 折叠
摘要
Abstract
Objective: To establish a high performance liquid chromatography(HPLC) for determining the content of benzalkonium bromide in oxymetazoline hydrochloride nasal drops.Methods:A Waters XBridgeTM Shield RP18 column(4.6 mm ×250 mm,5μm) was used with a mobile phase of 0.005 mol・L-1 ammonium acetate solution ( consisting of 10 mL triethylamine per 1000 mL, adjusted to pH5.0 by glacial acetic acid)-acetonitrile(40:60) at a flow rate of 1.0 mL・min-1.The detection wavelength was set at 214 nm, and the column temperature was 25 ( C.Results:The concentration of benzalkonium bromide in the range of 0.0109~0.327 mg・mL-1 showed good liner relation with the peak area (r=0.9995, n=6).The average recovery was 100.1%,RSD=1.0%,(n=9).Conclusions:The method can be used for the content determination of benzalkonium bromide in Oxymetazoline Hydrochloride Nasal Drops which pro-vides the advantage of simplicity, accuraty and repeatability.关键词
高效液相色谱法/盐酸羟甲唑啉/滴鼻液/苯扎溴铵/含量测定Key words
High performance liquid chromatography/Oxymetazoline hydrochloride/Nasal drops/Benzalkonium bromide/Content determination分类
医药卫生引用本文复制引用
赵晓頔,张肖宁,杨利红..HPLC法测定盐酸羟甲唑啉滴鼻液中苯扎溴铵的含量[J].中国药物评价,2016,33(3):133-135,3.
