中华灾害救援医学2016，Vol.4Issue(9)：482-485,4.DOI:10.13919/j.issn.2095-6274.2016.09.001
电针合谷穴对窒息大鼠心肺复苏后神经功能及生存率的影响
Effect of electroacupuncture at Hegu acupoints on survival and neurological function after cardiopulmonary resuscitation in asphyxial cardiac arrest rats
摘要
Abstract
Objective To investigate effect of electroacupuncture at Hegu acupoints on survival rate and neurological function after cardiopulmonary resuscitation in asphyxia1 cardiac arrest rats.Methods Asphyxial cardiac arrest was induced with asphyxia by clamping the tracheal tubes. Male Sprague-Dawley rats were randomly divided into five groups: (1) cardiopulmonary resuscitation group (group C), with right intracerebroventricular injection of 5μl physiologic saline; (2) atropine methyl nitrate administered group (group A), with right intracerebroventricular injection of atropine methyl nitrate (5μg/kg); (3) electroacupuncture administered group (group E), with electroacupuncture at Hegu acupoints and right intracerebroventricular injection of physiologic saline; (4) electroacupuncture plus atropine methyl nitrate group (group EA), with electroacupuncture at Hegu acupoints and right intracerebroventricular injection of atropine methyl nitrate. 8 rats in each group were used for neural function observation. They were subjected to 5 minutes of asphyxial cardiac arrest, and then neurological deficits were evaluated and scored at 24, 48 and 72 h respectively after restoration of spontaneous circulation (ROSC). 12 rats in each group were used for survival study. They were also subjected to 8 minutes of asphyxial cardiac arrest, and then survival conditions were observed every 2 h within 72 h after ROSC.Results At 24 h and 48 h after ROSC, the differences in neurological deficit scores (NDS) among all four groups was not statistically significant (χ2=3.828,P=0.281;χ2=3.728,P=0.292), but at 72 h after ROSC, NDS showed statistically significant differences among four groups. And then further pairwise comparison showed that NDS in group E was significantly higher than that in group C, and the difference was statiscally significant (P=0.015). Survival rate within 72 h of group C, group E, group A and group EA were 25% (3/12),66.67% (8/12), 16.67% (2/12) and 33.33% (4/12), respectively. Survival rate within 72 h of group E was higher than that of group C, the difference was statistically significant (χ2=3.874,P=0.049).Conclusions Electroacupuncture at Hegu acupoints can increase survival rate and improve neurological function after cardiopulmonary resuscitation in asphyxial cardiac arrest rats, which is probably related with activating central cholinergic system.关键词
电针/窒息/心肺复苏/神经功能/生存率Key words
electroacupuncture/asphyxia/cardiopulmonary resuscitation/neurological function/survival rate分类
医药卫生引用本文复制引用
房巍,梁小燕,庄永敬,曹云飞,周黎明..电针合谷穴对窒息大鼠心肺复苏后神经功能及生存率的影响[J].中华灾害救援医学,2016,4(9):482-485,4.基金项目
广东省中医药局科研课题（20151039）；全军“十二五”医学创新专项课题（13CXZ045）；2014年度全军医学科技青年培育项目计划 （）
