现代农业科技Issue(17)：251-252,255,3.
高抗菌活性纳豆芽孢杆菌的诱变筛选
Mutation Breeding of Antimicrobial Bacillus natto
杨晓志 1刘丹1
作者信息
- 1. 江苏农牧科技职业学院，江苏泰州 225300
- 折叠
摘要
Abstract
In this study,four strains of Bacillus natto were separated and purificated from the natto powders.In order to improve the antibacterial substance production,the four strains was irradiated by UV and NTG.The results showed that when Bacillus natto be irradiated by UV for 80 s or be treated by 0.4 mg/mL NTG,the lethality rates were 83%and 87% respectively.After treatment with compound mutation,60 stains was screened out according to the growth rate of bacteria,diameter of colonies and inhibitted bacteria circles.After secondary screening with slip method and cylinder-plate method,a mutant strain(L-23)was selected,which can generate a 27 mm inhibitted bacteria circles and it has a good stability in generation.关键词
纳豆芽孢杆菌/抑菌/诱变/筛选Key words
bacillus natto/antibacterial/mutation/screen分类
化学化工引用本文复制引用
杨晓志,刘丹..高抗菌活性纳豆芽孢杆菌的诱变筛选[J].现代农业科技,2016,(17):251-252,255,3.
