安徽农业科学2016，Vol.44Issue(25)：7-9,20,4.
盐藻中叶绿素的提取方法比较及条件优化
Comparison and Optimization of the Methods for Extracting Chlorophyll from Dunaliella salina
摘要
Abstract
Objective] To determine the effective method and optimal conditions for extracting chlorophyll from Dunaliella salina.[ Method] On the basis of the single factor investigation of optimal conditions for extracting chlorophyll from D.salina by ethanol, the whole optimal con-ditions were obtained by means of orthogonal design .[ Result] The optimized conditions were 85% ethanol, 12 min extraction time and 40 ℃extraction temperature.The maximum extraction rate was 10.80 mg/g wet weight by ethanol, and it was 15.5% higher than that by acetone extract.[ Conclusion] More chlorophyll could be extracted from D.salina by ethanol, and ethanol extraction was safe, non-polluting and less time consuming.This result will provide experimental basis and technology reference for using ethanol to extract and determine chlorophyll content or producing chlorophyll from marine D.salina in industrial manufacture.关键词
盐藻/叶绿素/提取条件/优化Key words
Dunaliella salina/Chlorophyll/Extraction conditions/Optimization分类
农业科技引用本文复制引用
杨代宇,穆富香,白飞妮,于媛,杨海波..盐藻中叶绿素的提取方法比较及条件优化[J].安徽农业科学,2016,44(25):7-9,20,4.基金项目
2016年辽宁省大学生创新训练项目（201611258000010）；2015年大连大学大学生创新创业训练计划项目（2015007）。 （）
