北京中医药大学学报2016，Vol.39Issue(9)：744-749,6.DOI:10.3969/j.issn.1006-2157.2016.09.009
基于神经保护探讨补阳还五汤抗脑缺血损伤的多靶点作用机制
Multi-target neuroprotective effect of Buyang Huanwu Decoction against cerebral ischemia injury
摘要
Abstract
Objective To explore the multi-target mechanism of neuroprotective effect of Buyang Huan-wu Decoction (BYHWD)against cerebral ischemia injury by using molecular docking.Methods 230 ingredients and metabolites of herbal Chinese medicines of BYHWD were collected and docked with seven active binding sites of five glutamate receptors.The potential anti-cerebral ischemia injury active com-pounds were screened and the molecular mechanism of the active ingredients was analyzed using molecu-lar docking.Results Calycosin,formononetin,perlolyrine and lactiflorin,main compounds of BYH-WD,interacted with two or more binding sites of glutamate receptors,which showed that BYHWD’s neu-roprotective effect against cerebral ischemia in the way of multiple compounds working on multiply targets and multiply sites.Majority of components from the chief drug Radix Astragali (Huangqi)and minority of components from deputy drug Radix Angelicae Sinensis (Danggui)and assistant herbs Rhizoma Chua-nxiong,Radix Paepmoae Rubra (Chishao),and Flos Carthami (Honghua),consistent with the combi-nation principles of BYHWD.Conclusion The multi-target neuroprotective effect of BYHWD against cerebral ischemia injury was studied at the molecular level,which can guide the application of BYHWD in clinic.Meanwhile,this study also can provide reference for discovering novel traditional Chinese medi-cines to treat cerebral ischemia injury.关键词
补阳还五汤/神经保护/脑缺血损伤/分子对接/多靶点Key words
Buyang Huanwu Decoction/neuroprotective/cerebral ischemia Injury/molecular docking/multi-target分类
医药卫生引用本文复制引用
路芳,蒋芦荻,陈艳昆,雒刚刚,刘芳,李贡宇,张燕玲..基于神经保护探讨补阳还五汤抗脑缺血损伤的多靶点作用机制[J].北京中医药大学学报,2016,39(9):744-749,6.基金项目
国家自然科学基金资助项目（No．81173522，No．81430094，No．81573831），北京市教育委员会共建资助项目，北京青年英才计划资助项目 （）
