森林与环境学报2016，Vol.36Issue(4)：409-415,7.DOI:10.13324/j.cnki.jfcf.2016.04.005
鳄梨组织培养繁殖体系优化研究
Regeneration system establishment of Persea americana
摘要
Abstract
To meet the need of Persea americana tissue culture, biotechnology was used to provide substantial good quality tissue culture seedlings, which is significant for the industry development and applic ation. Taking stem segments of mutant strains of P. americana as explants, optimum medium for sterile strains establishment, latent bud germination, bud multiplication, and rooting were selected. The results revealed that:the optimum medium of sterile strains establishment was WPM+1.0 mg•L-16￣BA+0.3 mg•L-1 NAA+0.5 mg•L-1ZT+2% sugar+0.2% activated carbon+0.65% agar; the optimum medium of latent bud germination was WPM+2.0 mg•L-16￣BA+0.5 mg•L-1NAA+1.0 mg•L-1ZT+2% sugar+0.2% activated carbon+0.65% agar, with a bud induction rate to 54%; the optimum medium of bud multiplication was WPM+3.0 mg•L-16￣BA+0.3 mg•L-1NAA+1.0 mg•L-1ZT+2% sugar+0.2%activated carbon+0.65% agar, with a reproduction rate up to 3.70;the optimum medium of root culture was 1/2 WPM+0.5 mg•L-1 IBA+0.5 mg•L-1 NAA+2% sugar+0.2% activated carbon+0.65% agar, with a rooting rate of 90%. The survival rate was 96% after transplanted for 60 d. The culture formula in the research can produce complete plantlet and gain lots of high quality seedlings in a short term.关键词
鳄梨/茎段/潜伏芽/组织培养Key words
Persea americana Mill./stem section/latent bud/tissue culture分类
农业科技引用本文复制引用
何碧珠,吴沙沙,兰思仁..鳄梨组织培养繁殖体系优化研究[J].森林与环境学报,2016,36(4):409-415,7.基金项目
福建省特色花卉品种创新与种苗设施繁育产业化工程(2014S1477－7)。 （2014S1477－7）
