福建农林大学学报（自然科学版）2016，Vol.45Issue(5)：529-535,7.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2016.05.009
桉树根际土壤解磷细菌的分离、筛选及其解磷效果
Isolation, screening of phosphate solubilizing capacity of phosphate solubilizing bacteria in Eucalyptus species
摘要
Abstract
To develop microbial compound fertilizer for Eucalyptusplantation, rhizosphere phosphorus ( P ) bacteria were separated and selected from the soil of Eucalyptus salignai, E. Dunnii and E. urophglla×grandis by selective medium. Then strains with most stable solubilizing capacity were further screened. Results showed that there existed about 2. 23 × 104~4. 17 × 104 cfu · g-1 organic phosphate-solubilizing bacteria and 2.05×104~4.00×104 cfu·g-1 inorganic phosphate-solubilizing bacteria in 3 kinds of forest soils. The quantity of bacteria from different stands in a descending order was Eucalyptus saligna, E. urophglla×E.grandis and E. Dunnii. Phosphate solubilizing capacities varied significantly among 12 strains of organic phosphate-solubilizing bacteria and 14 strains of in-organic phosphate-solubilizing bacteria (P<0.05), with available P absorbed by inorganic phosphate-solubilizing bacteria ranging from 55.854 to 367.169 g·mL-1 , and available P dissolved by organic phosphate-solubilizing bacteria ranging from 11.374 to 30. 330 g·mL-1 . There was significantly negative relationship between pH of culture solution and the content of inorganic P solubi-lized by inorganic phosphate-solubilizing bacteria, though pH was not correlated with the content of organic P dissolved by organic phosphate-solubilizing bacteria. Among 26 strains, strain P7 demonstrated the highest capacity in solubilizing inorganic P and strain YP8 was the most efficient in dissolving organic P, which were recommended as microbial fertilizer for Eucalyptus species.关键词
桉树/解磷细菌/溶磷能力/有效磷Key words
Eucalyptus/phosphate solubilizing bacteria/phosphate solubilizing capacity/available phosphorous分类
农业科技引用本文复制引用
徐欢,俞新玲,林勇明,吴承祯,谢安强,陈灿,李键,洪滔..桉树根际土壤解磷细菌的分离、筛选及其解磷效果[J].福建农林大学学报（自然科学版）,2016,45(5):529-535,7.基金项目
福建省科技厅重大专项项目(2012NZ01) （2012NZ01）
教育部博士点学科专项基金资助项目(20123515110011). （20123515110011）
