福建农林大学学报（自然科学版）2016，Vol.45Issue(5)：556-560,5.DOI:10.13323/j.cnki.j.fafu(nat.sci.).2016.05.013
NtMAP70基因在烟草抗马铃薯Y病毒过程中的作用
Role of NtMAP70 in the resistance of tobacco to potato virus Y infection
摘要
Abstract
To elucidate the mechanism of microtubule-associated protein 70 ( MAP70) on combating Potato virus Y ( PVY) , full-length NtMAP70 was firstly isolated, and transcriptional expression levels of NtMAP70 in different organs and growing periods were measured by real-time quantitative PCR. Then NtMAP70 gene silencing vector, based on geminivirus satellite, was introduced into Agrobacterium tumefaciens strain EHA105. And Agrobecterium harboring recombinant silencing vector and Tomato yellow leaf curl China virus ( TYLCCNV) infectious clone were co-agroinoculated into tobacco plants. Results showed that NtMAP70 was expressed in all organs and growing periods, among which the expression levels was highest in the root. Compared with the control, PVY infec-tion rate was lower in tobacco plants that inoculated with silenced gene so as a reduced level of PVY. To summarize, silenced Nt-MAP70 gene is very likely to suppress the proliferation of PVY and retard its activity.关键词
NtMAP70/马铃薯Y病毒/烟草/抗性Key words
NtMAP70/PVY/tobacco/resistance分类
农业科技引用本文复制引用
詹琳琳,郭玉双,蔡健宇,武晓云,杨靓..NtMAP70基因在烟草抗马铃薯Y病毒过程中的作用[J].福建农林大学学报（自然科学版）,2016,45(5):556-560,5.基金项目
国家自然科学基金(31360431). （31360431）
