广东工业大学学报2016，Vol.33Issue(5)：77-82,6.DOI:10.3969/j.issn.1007-7162.2016.05.014
响应面优化弹性蛋白酶酶解罗非鱼下脚料的工艺研究
Optimization of Enzymolysis of Tilapia Waste with Response Surface Methodology by Elastase
摘要
Abstract
The head, bones, and other raw waste of Tilapia are hydrolyzed by elastase, and response sur-face analysis method is used to optimize the enzymatic processes.The enzyme concentration, tempera-ture, pH, solid to liquid ratio, time and other factors are discussed to analyze the effect of hydrolysis based on the degree of hydrolysis and peptide content.The results show that the optimum conditions of elastase hydrolysis:enzyme concentration 0.553 mg/g, temperature 41.94 ℃, time 3.31 h, pH natu-ral, solid-liquid ratio 1∶5, the degree of hydrolysis and the peptide content is separately 18.09% and 0.90 mg /ml in this condition.关键词
罗非鱼下脚料/响应面分析/弹性蛋白酶/水解度/多肽含量Key words
Tilapia waste/response surface methodology/elastase/hydrolysis/peptide分类
轻工纺织引用本文复制引用
瞿叶辉,丁利君,彭文媛,刘珊珊,黄金,成晓玲..响应面优化弹性蛋白酶酶解罗非鱼下脚料的工艺研究[J].广东工业大学学报,2016,33(5):77-82,6.基金项目
广东省教育部产学研合作重大专项（2013A090100009）． （）
