军事医学2016，Vol.40Issue(9)：697-702,6.DOI:10.7644/j.issn.1674-9960.2016.09.001
CDC42在HBx介导肝细胞恶性转化中作用的定量蛋白质组学研究
Quantitative proteomics of CDC42 in HBx-mediated cellular transformation
摘要
Abstract
Objective To identify the mediators of CDC42 signaling pathway involved in hepatitis B virus X protein (HBx)-mediated cellular transformation.Methods The mass defect-based pseudo-isobaric dimethyl labeling method (pIDL)was used to detect the differentially expressed proteins with a deficiency of CDC42.Furthermore,we conducted a gene ontology (GO)of differentially expressed proteins.Results and Conclusion We totally qualified 3409 proteins and found 220 differentially expressed proteins.Palladin,formin-like 1 (FMNL1)and keratin-19,which were implicated in cytoskeleton organization,were down-regulated with the deficiency of CDC42.Our results have provided candidate genes and proteins that may play an important role in HBx-mediated cellular transformation.关键词
定量蛋白质组学/细胞骨架/肝细胞癌/CDC42/基因缺失Key words
quantitative proteomics/cytoskeleton/hepatocellular carcinoma/CDC42/gene deletion分类
医药卫生引用本文复制引用
徐永茹,齐英姿,徐平,李湘萍,徐锋..CDC42在HBx介导肝细胞恶性转化中作用的定量蛋白质组学研究[J].军事医学,2016,40(9):697-702,6.基金项目
国家973计划资助项目（2011CB910600，2013CB911200）；国家自然科学基金资助项目（31470809，31170780）；北京市自然科学基金资助项目（5152008）；国家重点实验室基金资助项目 （）
