军事医学2016，Vol.40Issue(9)：707-709,3.DOI:10.7644/j.issn.1674-9960.2016.09.003
组蛋白甲基转移酶SET7的原核表达及纯化鉴定
Prokaryotic expression and purification of SET7 with GST-tag
摘要
Abstract
Objective To purify and prokaryotically express the histone methyltransferase SET7.Methods The coding sequence of full length SET7 was amplified from breast library by PCR and cloned into the pGEX-KG vector.The correct recombinant plasmid was introduced into E.coli.The expressed protein was identified by SDS-PAGE.Results DNA sequencing indicated the SET7 was constructed.GST-SET7 fusion protein was identified by SDS-PAGE.Conclusion The prokaryotically expressed protein of GST-SET7 is obtained,which will falilitate further study of SET7 function.关键词
SET7基因/原核表达/纯化/乳腺肿瘤Key words
SET7 gene/prokaryotic expression/purification/breast neoplasms分类
医药卫生引用本文复制引用
刘婕,付正香,张亚楠,姜阳,陈志达,丁丽华,叶棋浓..组蛋白甲基转移酶SET7的原核表达及纯化鉴定[J].军事医学,2016,40(9):707-709,3.基金项目
国家自然科学基金资助项目 （）
