军事医学2016，Vol.40Issue(9)：710-712,732,4.DOI:10.7644/j.issn.1674-9960.2016.09.004
艰难梭菌谷氨酸脱氢酶的表达及抗原性分析
Expression and antigenicity analysis of Clostridium difficile glutamate dehydrogenase
摘要
Abstract
Objective To construct prokaryotic expression vectors for glutamate dehydrogenase(GDH)of Clostridium difficile(CD),and express recombinant GDH in Escherichia coli,and identify its antigenicityed.Methods The entire gene of GDH was cloned from ATCC43255 genome DNA.The recombinant antigens were expressed in E.coli with IPTG induction and purified by Ni-NATBeads.The antigenicity was detected using CD Qick Chek Complete dual-antigen EIA. Results Prokaryotic expression vectors of CD GDH were constructed successfully.The antigen could be identified by specific anti-GDH antibodies.Conclusion The GDH antigen can be used to prepare corresponding antibodies,which facilitate the development of immunoassay for CD GDH.关键词
艰难梭菌/谷氨酸脱氢酶/原核表达Key words
Clostridium difficile/glutamate dehydrogenase/prokaryotic expression分类
医药卫生引用本文复制引用
王建霞,杨锡琴,金大智,黄忱,罗芸,宋晓国,王宏伟,冯晓燕,张贺秋..艰难梭菌谷氨酸脱氢酶的表达及抗原性分析[J].军事医学,2016,40(9):710-712,732,4.基金项目
全军后勤科研计划资助项目（CWS13J049，AWS15J006）；国家自然科学基金资助项目（81471998）；国家卫生计生委科学研究基金－浙江省医药卫生重大科技计划 （）
