军事医学2016，Vol.40Issue(9)：722-724,745,4.DOI:10.7644/j.issn.1674-9960.2016.09.007
亚洲副溶血弧菌临床菌株的基于碱基序列和肽链序列的多位点序列分型研究
Population analysis of clinical Vibrio parahaemolyticus strains from Asia based on multilocus sequence typing and AA-multilocus sequence typing
摘要
Abstract
Objective To find out more about the population structure and clonal complex of clinical Vibrio parahaemolyticus strains in Asia.Methods Clinical Vibrio parahaemolyticus strains data were screened in Asia with complete ST and pST types from PubMLST public database,their subgroup and complex were analyzed,and the minimum spanning tree based on ST and pST types respectively was completed.Results From the database,341 items of ST and pST types of Asian clinical Vibrio parahaemolyticus strains were screened,including 157 ST,most of which were of ST3 type.Totally 214 items of data came from China (including Hong Kong and Taiwan),and covered 133 ST,most of which were of ST3 type.eBURST software was used and 17 groups and 94 singletons were found.Software STRUCTURE analysis showed that the appropriate subset number of clinical V.parahaemolyticus strains in Asia was 7,and that the average distance between samples in each subgroup was 0.9113.Conclusion Clinical V.parahaemolyticus strains in Asia show high diversity and can be subdivided into seven subgroups.ST3 type is dominating when multilocus sequence typing(MLST)is used and pST2 type is the majority by AA-MLST typing.关键词
副溶血弧菌/基于碱基序列的多位点序列分型/基于肽链的多位点序列分型/克隆复合体Key words
Vibrio parahaemolyticus/multilocus sequence typing/peptide based multilocus sequence typing/clonal complex分类
医药卫生引用本文复制引用
徐嘉良,杜小莉,刘俊含,阚飙,卢昕..亚洲副溶血弧菌临床菌株的基于碱基序列和肽链序列的多位点序列分型研究[J].军事医学,2016,40(9):722-724,745,4.基金项目
国家自然科学基金资助项目（31400161，81401715）；北京工商大学青年教师科研启动基金资助项目 （）
