军事医学2016，Vol.40Issue(9)：725-728,4.DOI:10.7644/j.issn.1674-9960.2016.09.008
紫外线对大肠杆菌的损伤机制研究
Mechanism of Escherichia coli injury under UV disinfection
摘要
Abstract
Objective To study the inactivation kinetics and injury mechanism of Escherichia coli under UV disinfection in drinking water.Methods The inactivation kinetics of E.coli ATCC 25922 was determined by plate count methods in the UV disinfection experiment.The morphology,cell membrane permeabilization and injury of biological macromolecules were observed under a transmission electron microscope (TEM).The rate of ONPG hydrolysis and changes in activities of antioxidant enzymes were observed Raman spectroscopy.Results the changes of damaged cells involved morphological damage such as loss of the structural integrity of the wall and membrane,condensation of cellular material and leakage of significant amounts of cytoplasmic material,a more than four-fold increase of cell membrane permeabilization and damage to the structure of protein,nucleic acids and phospholipid.Conclusion UV disinfection can lead to a multi-target damage.关键词
紫外线/消毒/大肠杆菌/损伤菌/光谱分析,拉曼/显微镜检查,电子,透射Key words
ultraviolet rays/disinfection/Escherichia coli/bacterial injury/spectrum analysis,Raman/microscopy, electron,transmission分类
医药卫生引用本文复制引用
李静,王大宁,曲红梅,邱志刚,尹静,李君文,金敏..紫外线对大肠杆菌的损伤机制研究[J].军事医学,2016,40(9):725-728,4.基金项目
国家自然科学基金资助项目 （）
