畜牧与饲料科学2016，Vol.37Issue(9)：1-3,21,4.
不同剂量环磷酰胺对小鼠免疫功能抑制作用的研究
Immunosuppression Effect of Different Doses of Cyclophosphamide on Mice
摘要
Abstract
The aims of the present study were to observe the immunosuppression effect of cyclophosphamide with different dosage regimen on mice and to explore the method for establishing immunosuppression model induced by cyclophosphamide in mice. Single or multiple doses of cyclophosphamide were intraperitoneally injected in mice. The immune organ index, serous level of alanine aminotransferase, aspartate aminotransferase, urea nitrogen and creatinine, blood leukocyte count, and splenic lymphocyte transformation were determined to evaluate the effect of cyclophosphamide on immunity and hepatic and renal function of mice. The results showed that both the multiple administration of 10, 20, 30, 40 mg/ (kg·BW) of cyclophosphamide for consecutive seven days respectively and single administration of 150 mg/ (kg·BW) of cyclophosphamide exhibited immunosuppression effect on mice and caused the changes of indicators associated with hepatic and renal function;the immunosuppression effect produced by multiple administration with lower dosage of cyclophosphamide was superior to that produced by single administration with higher dosage, and the immunosuppression effect was improved with the increase of dosage under multiple administration mode. The combined data suggest that the immunosuppression model in mice can be established by multiple administration of 30-40 mg/(kg·BW) of cyclophosphamide for consecutive seven days.关键词
小鼠/环磷酰胺/给药剂量/给药方式/免疫抑制Key words
mice/cyclophosphamide/administration dosage/dosage regimen/immunosuppression分类
农业科技引用本文复制引用
李璐,黄宏业,韦现色,王秋华,杨善忠,何家康,杨剑,胡庭俊..不同剂量环磷酰胺对小鼠免疫功能抑制作用的研究[J].畜牧与饲料科学,2016,37(9):1-3,21,4.基金项目
国家自然科学基金资助项目（31560708）；高等学校博士学科点专项科研基金博导类资助课题（20134501110004）；南宁市科学研究与技术开发计划项目（合同编号20155181）。 （）
