水生态学杂志2016，Vol.37Issue(5)：82-86,5.DOI:10.15928/j.1674-3075.2016.05.012
三氯生对红白鲫的急性毒性及遗传毒性研究
Acute and Genetic Toxicity of Triclosan to Red-White Crucian Carp
摘要
Abstract
Triclosan (TCS )is a common antibacterial agent widely used in personal care products,household cleaning products and medical supplies.As a result,it is now frequently detected in various environmental media, organisms and humans.Studies have showed that TCS is not readily biodegraded and produces a variety of toxic effects,including lethality,immunotoxicity,indirect carcinogenicity and genotoxicity after bioaccumulation.Verte-brate organ systems affected include the heart,liver,kidney,muscle,thyroid gland and thymus gland.Few studies have been carried out on the genotoxicity of TCS to fish.In this study,the acute and genetic toxicity of TCS to red-white crucian carp were determined to provide data for evaluating the effect of TCS on fish genetics and ecology. Red-white crucian carp with body length of 6.8-7.5 cm were acclimated for one week in a glass aquarium (58 cm ×28 cm ×36 cm).After acclimation,two trials were conducted under the following conditions:temperature,(18 ±3)℃;dissolved oxygen,>5.0 mg/L;pH,7.0 ±0.2.In the acute toxicity test,the carp were randomly as-signed to six TCS concentration groups (600.0,690.0,793.5,912.5,1 049.4 and 1 206.8 μg/L),a blank control group and a DMSO (0.1%)solvent control group for a 96h semi-static exposure without feeding and abnor-mal activity,body color change and mortality were recorded.In the genotoxicity test,the carp were randomized into three TCS concentration groups (600.0 μg/L,690.0 μg/L,793.5 μg/L)and two control groups for a 14 d semi-static exposure with daily feeding and the micronucleus rate and abnormal nuclei rate of blood erythrocytes were recorded.During the two trials,all tests were run in triplicate with 10 fish in each group and the solutions were changed each 24 hours.Results show that the 96 h median lethal concentration (96 h LC50 )of TCS was 1 1 1 1 .9 μg/L and the safe concentration was 1 1 1 .19 μg/L.After a 14 day exposure,abnormalities among blood e-rythrocytes nuclei increased significantly.Abnormalities of blood erythrocyte nuclei presented as small and large mi-cronuclei,double micronuclei,unequally constricted nuclei,abnormally positioned nuclei,vacuolated nuclei, double nuclei,irregular nuclei,bulging nuclei,notched nuclei and fragmented nuclei.Among these,single and double micronuclei were the major abnormalities and bulging nuclei,notched nuclei and vacuolated nuclei were also commonly observed.There were no significant differences in the abnormal nuclei and micronucleus formation rates in the blank group and solvent control group (P>0.05 ),but abnormalities in the TCS treatment groups (600.0 μg/L,690.0 μg/L,793.5 μg/L)were significantly higher than in the two control groups (P<0.01). Triclosan induced the formation of abnormal nuclei in blood erythrocytes,which occurred at significantly higher rates and displayed the classic dose-effect relationship.Triclosan produces genotoxicity in red-white crucian carp and the effect increases with TCS concentration.关键词
三氯生/红白鲫/急性毒性/遗传毒性Key words
triclosan/red-white crucian carp/acute toxicity/genetic toxicity分类
资源环境引用本文复制引用
刘飞,刘旭昊,王凡,蒋春春,种贺林,曹保红,张振华..三氯生对红白鲫的急性毒性及遗传毒性研究[J].水生态学杂志,2016,37(5):82-86,5.基金项目
NSFC-河南人才培养联合基金（U1504303）。 （）
