天津中医药大学学报2016，Vol.35Issue(5)：322-326,5.DOI:10.11656/j.issn.1673-9043.2016.05.10
炮制时间对杜仲指标成分含量及药代动力学影响研究
Influence of processing time on Eucommia cortex and its pharmacokinetics study
摘要
Abstract
Objectives] To develop a set of LC-MS/MS method for the simultaneous determination the compound of geniposide, geniposidic acid, aucubin and pinoresinol diglucoside in the different processed barks of Eucommia and for the analysis of biolcgical samples which contain the compound of geniposide, geniposidic acid, aucubin and pinoresinol diglucoside from the rats' oral administration of the Eucommia. [Methods] The Eucommia was processed with saline water according to Chinese pharmacopoeia. In the parch processing course, samples were randomly collected at 0,1,2 and 4 h respectively. Validation of a high performance liquid chromatography tandem triple quadrupole mass spectrometry LC-MS/MS (QQQ) method, with the strychni prime as internal standard, was used for the determination of the four target compounds in rats' plasma to study the pharmacokinetics of Eucommia. [Results] The determination methods with good precision and stability were suitable for the assay of Eucommia in the biological samples. It is reasonable to control the process time for Eucommia at 2 hours. [Conclusion] Additionally, these constituents demonstrated good pharmacokinetic properties in the body. The pharmacokinetics parameters in our research provided with information for the clinical application.关键词
杜仲/炮制/LC-MS/MS/药代动力学Key words
Eucommia/processing/LC-MS/MS/pharmacokinetics分类
医药卫生引用本文复制引用
张影月,韩亚亚,郝佳,王涛,刘二伟..炮制时间对杜仲指标成分含量及药代动力学影响研究[J].天津中医药大学学报,2016,35(5):322-326,5.基金项目
高等学校博士学科点专项科研基金联合资助课题（20131210120010）；国家科技重大专项“孵化基地”课题（2013ZX09401004）；天津市高等学校创新团队（TD12-5036， TD12-5033）；天津市抗癌重大专项项目（12ZCDZSY17000）。 （）
