烟草科技2016，Vol.49Issue(9)：1-7,7.DOI:10.16135/j.issn1002-0861.2016.0138
烟草E3泛素连接酶NtHOS1a和NtHOS1b基因的克隆与表达分析
Cloning and expression analysis of two E3 ubiquitin ligase genes NtHOS1a and NtHOS1bin tobacco(NicotianatabacumL.)
摘要
Abstract
To identify the genes related to the regulation of early flowering at low temperature in tobacco, two full-length cDNAs encoding different E3 ubiquitin ligase genes (NtHOS1a and NtHOS1b) were cloned from Nicotiana tabacum by bioinformatics, RT-PCR and SMART RACE technologies. GenBank accession numbers of NtHOS1a and NtHOS1b were KU558689 and KU558690, respectively. The putative full-length cDNA sequences of NtHOS1a and NtHOS1b were 3 599 bp and 3 578 bp, and these two genes encoded the predicted polypeptide of 963 and 954 amino acids, respectively. Amino acids identity between NtHOS1a and NtHOS1b was 95%. Putative polypeptide in the two genes matched Arabidopsis thaliana HOS1 (NP_181511) polypeptide sequence with the identity of 51% and 50%,respectively. The two proteins shared highly conserved N-terminal RING domains of E3 ubiquitin ligases. qRT-PCR showed that under normal growing condition, NtHOS1a and NtHOS1b transcripts accumulated to a high level in root, stem and leaves. After 10 days of 12 ℃ treatment, the expression levels of the two genes were decreased to different degrees, this suggested that NtHOS1a and NtHOS1b expressions in tobacco root, stem and leaves were negatively regulated by low temperature.关键词
烟草/E3泛素连接酶(HOS1)/基因克隆/表达分析Key words
Nicotiana tabacum/E3 ubiquitin ligase(HOS1)/Gene cloning/Expression analysis分类
轻工纺织引用本文复制引用
林世锋,余婧,王志红,任学良,王仁刚..烟草E3泛素连接酶NtHOS1a和NtHOS1b基因的克隆与表达分析[J].烟草科技,2016,49(9):1-7,7.基金项目
中国烟草总公司重点项目“品种抗旱鉴定体系研究及抗旱烤烟品种选育”（110201302004）；中国烟草总公司贵州省公司科技项目“贵州烤烟新品种杂交选育研究”（2011-3047）；烟草行业分子遗传重点实验室专项经费项目“烟草抗早花抗病毒调控基因筛选和种质创制”（110201403018）。 （）
