中国康复理论与实践2016，Vol.22Issue(10)：1117-1120,4.DOI:10.3969/j.issn.1006-9771.2016.10.001
运动预处理对脑缺血再灌注大鼠缺血半暗区细胞凋亡及相关蛋白P53表达的影响
Effect of Exercise Preconditioning on Apoptosis and Expression of P53 in Ischemic Penumbra in Rats after Cere-bral Ischemia-Reperfusion
摘要
Abstract
Objective To explore the effect of exercise preconditioning on apoptosis and expression of P53 protein in ischemic penum-bra in rats after cerebral ischemia-reperfusion. Methods Thirty-six Sprague-Dawley rats were randomly divided into sham group, model group and exercise preconditioning group, with twelve rats in each group. The middle cerebral artery occlusion (MCAO) model was estab-lished with modified Longa's method. TUNEL method was used to observe the apoptosis of neural cells in the ischemic penumbra. Western blotting was used to detect the expression of P53 protein in the ischemic penumbra. Results Twenty-four hours after cerebral ischemia-reper-fusion, the number of TUNEL positive cells was more in the model group than in the sham group (P<0.01), and was less in the exercise pre-conditioning group than in the model group (P<0.01). The expression of P53 protein was higher in the model group than in the sham group (P<0.01), and was lower in the exercise preconditioning group than in the model group (P<0.01). Conclusion Exercise preconditioning coud down-regulate the expression of P53 protein in the ischemic penumbra, and inhibit the apoptosis of cortical cells.关键词
脑缺血再灌注/运动预处理/细胞凋亡/P53/大鼠Key words
cerebral ischemia-reperfusion/exercise preconditioning/apoptosis/P53/rats分类
医药卫生引用本文复制引用
吴孝军,叶涛,李宏玉,姜云飞,张中伟,朱路文..运动预处理对脑缺血再灌注大鼠缺血半暗区细胞凋亡及相关蛋白P53表达的影响[J].中国康复理论与实践,2016,22(10):1117-1120,4.基金项目
1.国家自然科学基金项目(No.81503666)；2.黑龙江省自然科学基金项目(No.QC2015103)；3.哈尔滨市科技创新人才专项基金项目(青年后备人)(No.2014RFQGJ150)；4.黑龙江中医药大学研究生科研创新项目(No.2015-33)。 （No.81503666）
