中国康复理论与实践2016，Vol.22Issue(10)：1145-1150,6.DOI:10.3969/j.issn.1006-9771.2016.10.007
超早期高压氧治疗对脊髓全横断损伤大鼠股骨骨钙、骨生物力学及骨胶原的影响
Effect of Ultra-early Hyperbaric Oxygenation on Bone Calcium, Biomechanical Properties and Bone Collagen of Femur in Rats with Complete Spinal Cord Transection Injury
摘要
Abstract
Objective To explore the effect of ultra-early hyperbaric oxygenation (HBO) on bone calcium, biomechanical properties and bone collagen of femur in rats with complete spinal cord transaction. Methods A total of 75 Sprague-Dawley rats were randomly divided in-to sham group (n=15), model group (n=20) and HBO group (n=40). HBO group was divided into three hours group (HBO1 group, n=20) and twelve hours group (HBO2 group, n=20). All groups underwent laminectomy at T10, while the model group, HBO1 group and HBO2 group underwent complete spinal cord transection at the same level. Three hours and twelve hours after surgery, HBO1 group and HBO2 group received HBO, respectively, for three courses with ten days in a course. After treatment, the femoral biomechanical properties, bone calcium and hydroxyproline (Hyp) were determined. The morphology of bone trabecula and the bone collagen was observed with HE stain-ing and Masson triad color staining, respectively. Results After treatment, compared with the sham group, the femoral biomechanical proper-ties, the content of bone calcium and Hyp decreased in the model group (P<0.05);compared with the model group and HBO2 group, they in-creased in HBO1 group (P<0.05). The number of bone trabecula and the bone collagen decreased, and derangement and sparseness were ob-served in the model group;however, the changes were substantially mild in HBO1 group. Conclusion Ultra-early HBO could increase the content of bone calcium and Hyp of femur, improve the morphology of the femur bone collagen, and improve the femoral biomechanical properties in rats with complete spinal cord transection.关键词
脊髓全横断/高压氧/超早期/股骨/骨钙/骨生物力学/骨胶原/大鼠Key words
complete spinal cord transection/hyperbaric oxygenation/ultra-early stage/femur/bone calcium/bone biomechanical properties/bone collagen/rats分类
医药卫生引用本文复制引用
刘敏,伍贤平,童敏,伍西羽,周金平..超早期高压氧治疗对脊髓全横断损伤大鼠股骨骨钙、骨生物力学及骨胶原的影响[J].中国康复理论与实践,2016,22(10):1145-1150,6.基金项目
湖南省科技厅基金项目(No.2008SK3108)。 （No.2008SK3108）
