中国康复理论与实践2016，Vol.22Issue(10)：1151-1153,3.DOI:10.3969/j.issn.1006-9771.2016.10.008
PM2.5对支气管上皮细胞色素上皮衍生因子表达的影响
Effects of PM2.5 on Expression of Pigment Epithelium-derived Factor in Bronchial Epithelial Cells
摘要
Abstract
Objective To explore the effect of particulate matter (PM) 2.5 on the expression of pigment epithelium-derived factor (PEDF) protein in bronchial epithelial cells (BEAS-2B). Methods Human BEAS-2B were subcultivated, followed by low, medium and high concentrations of PM2.5 (25μg/ml, 50μg/ml, 100μg/ml) stimulation for 24 hours. The expression of PEDF protein in supernatant was ana-lyzed by enzyme-linked immunosorbent assay (ELISA), and the expression in BEAS-2B cells was detected by Western blotting. Results Compared with the control group, the expression of PEDF protein in supernatant and BEAS-2B cells induced by PM2.5 (25 μg/ml) in-creased, but no significance was found (t=-0.730, t=-1.840, P>0.05), and the expression induced by PM2.5 with the concentrations of 50μg/ml and 100μg/ml significantly increased (t>5.798, P<0.05). Conclusion PM2.5 with the concentrations of 50μg/ml and 100μg/ml could increase the expression of PEDF protein in a concentration-dependent manner both in supernatant and BEAS-2B cells.关键词
细颗粒物/支气管上皮细胞/色素上皮衍生因子Key words
particulate matter/bronchial epithelial cells/pigment epithelium-derived factor分类
医药卫生引用本文复制引用
朱成华,施丽君,张灵凤,计晓兰,杜强..PM2.5对支气管上皮细胞色素上皮衍生因子表达的影响[J].中国康复理论与实践,2016,22(10):1151-1153,3.基金项目
1.江苏省高等学校大学生实践创新训练计划项目(No.201410312015Z)；2.江苏省六大人才高峰项目(No.WSN-015)。 （No.201410312015Z）
