中国免疫学杂志2016，Vol.32Issue(10)：1450-1453,4.DOI:10.3969/j.issn.1000-484X.2016.10.009
吉西他滨对人非小细胞肺癌HCC827活性和凋亡的影响
Effect of Gemcitabine on viability and apoptosis of non-small cell lung cancer HCC827 in vitro
张婧婧 1赵庆伟 1杨景瑞 1段巨洪 1于海川1
作者信息
- 1. 河南省新乡医学院医学检验学院分子诊断与医学检验技术河南省协同创新中心，新乡 453003
- 折叠
摘要
Abstract
Objective:To observe the effect of Gemcitabine ( GEM) on the viability and apoptosis of non-small cell lung cancer HCC827 in vitro. Methods:The cell viability,apoptosis and cell cycle of HCC827 cells induced by Gemcitabine were detected with cell counting kit-8 assay (CCK-8),Annexin V-FITC/PI staining and flow cytometry. The expression of Bcl-2 protein of cells treated with GEM was examined by Western blot assay. Results: There was significant inhibition effect on HCC827 cells treated with 0. 1-1 000 ng/ml of GEM,which can promote the occurrence of HCC827 cell apoptosis and arrest cell in the S phrase. The apoptosis induced by GEM was accompanied with the down regulation of Bcl-2 protein. Conclusion: GEM can inhibit the cell viability and induce the HCC827 cell apoptosis and S phrase arrest. Its cell dead type was apoptosis,which was related with the expression of Bcl-2 protein.关键词
细胞凋亡/人非小细胞肺癌HCC827细胞/吉西他滨/Bcl-2蛋白Key words
Cell apoptosis/Human non-small cell lung cancer HCC827 cell/Gemcitabine/Bcl-2 protein分类
医药卫生引用本文复制引用
张婧婧,赵庆伟,杨景瑞,段巨洪,于海川..吉西他滨对人非小细胞肺癌HCC827活性和凋亡的影响[J].中国免疫学杂志,2016,32(10):1450-1453,4.
