中国免疫学杂志2016，Vol.32Issue(10)：1458-1461,4.DOI:10.3969/j.issn.1000-484X.2016.10.011
表没食子儿茶素没食子酸酯（EGCG）对免疫性肝损伤的保护作用及其机制研究
Study of protective effect of EGCG on immunological liver injury and relevant mechanism
摘要
Abstract
Objective:To investigate the protective effect of Epigallocatechin-3-gallate (EGCG) on triptolide (TP)-induced immunological liver injuries, and explore the relevant mechanisms of action. Methods: A total of 40 female C57BL/6 mice were randomly divided into four groups:control group,EGCG group,TP group and TP+EGCG group. The ALT levels in serum was examined by Reitman Frankel method. The activity of hepatic MDA,SOD and GSH was examined by spectrophotometry. HE staining was used to observed the changes of the hepatic histopathology. The hepatic IL-17,IL-6 levels was examined by ELISA and the expression of hepatic TLR4 protein was examined by Western blot. Results:The results showed that serum alanine aminotransaminase ( ALT) levels of TP group were obviously elevated (P<0. 005,vs normal control group) and serum ALT levels were obviously reduced in EGCG treatment group(P<0. 005,vs normal TP group). There were no significantly differences between EGCG group and control group (P>0. 05). Meanwhile,EGCG could ameliorate hepatic pathological damage. Furthermore,in TP group,the activity of malondialdehyde ( MDA) ,the expression of Toll-like receptor 4 (TLR4) protein and the contentration of hepatic interleukin (IL)-17,IL-6 were higher than normal control group ( P<0. 005 ) . On the contrary, the activity of superoxide dismutase ( SOD ) and restored glutathione ( GSH ) were significantly lower than normal control group ( SOD, P<0. 05;GSH, P<0. 005 ) . In EGCG treatment group, the expression of TLR4 protein and the concentration of MDA,hepatic IL-17 and IL-6 were lower than TP group ( TLR4,P<0. 05;MDA,P<0. 005;IL-17,P<0. 005;IL-6,P<0. 005). On the contrary,SOD and GSH were significantly higher than TP group (SOD,P<0. 05;GSH,P<0. 005). Conclusion:This study suggests that EGCG possesses hepatoprotective effect against TP-induced immunological liver injury through its anti-inflammatory and anti-oxidant actions.关键词
表没食子儿茶素没食子酸酯/雷公藤甲素/肝损伤/炎症因子/抗氧化作用Key words
EGCG/TP/Liver injury/Inflammatory cytokines/Antioxidant effect分类
医药卫生引用本文复制引用
于淑静,周莲娣,魏彩冰,孙宗良,张起辉,陶崑..表没食子儿茶素没食子酸酯（EGCG）对免疫性肝损伤的保护作用及其机制研究[J].中国免疫学杂志,2016,32(10):1458-1461,4.基金项目
本文为重庆市基础与前沿研究项目(cstc2014jcyjA10108)和中央高校基本业务费(CQDXWL-2014-Z007 （cstc2014jcyjA10108）
201310611045)。 （）
