中国免疫学杂志2016，Vol.32Issue(10)：1491-1495,5.DOI:10.3969/j.issn.1000-484X.2016.10.018
人DcR3表达载体的构建及验证
Over-expression vector construction of human DcR3 gene and its validation
摘要
Abstract
Objective:To construct the human DcR3 expression vector and verify its expression in vitro. Methods: 915 bp human DcR3 gene CDS was amplified from porcine lung tissues,and was cloned into eukaryotic expression vector pEF1a-IRES-DsRed-Express2 which show red fluorescence. And then pEF1a-IRES-DsRed-Express2-DcR3 was transfected into LX-2 cells by FuGene HD. Expression of mRNA and protein lever of Human DcR3 were detected by RT-PCR and Western blot. Results:The levels of DcR3 gene transcription and translation in the hepatic stellate cells were significantly increased after transfection with pEF1a-IRES-DsRed-Ex-press2-DcR3 by RT-PCR and Western blot analysis. Conclusion: DcR3 expression vector was successfully constructed and highly expressed in LX-2 cells.关键词
诱骗受体3/肝星状细胞/真核表达/克隆Key words
DcR3/LX-2/Eukaryotic expression/Clone分类
医药卫生引用本文复制引用
潘留兰,贾胜男,马静婷,太京华,金珍婧..人DcR3表达载体的构建及验证[J].中国免疫学杂志,2016,32(10):1491-1495,5.基金项目
本文为吉林省科学技术委员会面上基金课题(201115083)。 （201115083）
