中国免疫学杂志2016，Vol.32Issue(10)：1496-1502,7.DOI:10.3969/j.issn.1000-484X.2016.10.019
乙肝病毒感染不同阶段抗原肽特异性细胞毒性 T 淋巴细胞的初步研究
Preliminary study on antigen specific CD8+T cells at different stages of HBV infection
摘要
Abstract
Objective:To clarify the differences of host immune responses at different stages of HBV infection. Methods:We constructed three HLA-A*0201/HBV tetramers with immunodominant epitopes of core18-27,polymerase 575-583 and envelope 335-343,and analyzed antigen specific CD8+ T cells and the expression of CD127 in peripheral blood mononuclear cells ( PBMCs) from patients infected with HBV using these HLA-A*0201/HBV tetramers. Results: The frequencies and expansion ability of antigen specific CD8+ T cells in most self-limited HBV infected individuals were higher than that in chronically HBV infected patients. In low copy period the frequencies of antigen specific CD8+ T cells were similar to those in immune clearance phase at a high viral load and liver damage and in immune clearance phase, which had no significant correlation with virus quantitation and ALT level. In chronic infection the ability of antigen specific CD8+ T cells proliferation was inversely proportional to the viral titer. In most self-limited HBV infected individuals the IFN-γsecretion functions of antigen specific CD8+ T cells were higher than in chronic infection,but in immune tolerance phase these cells lost the ability. HBsAg level was different at different stages after HBV infection:it was highest in immune tolerance phase,but in immune clearance phase,activity period and low copy period the correlation with HBV DNA replication gradually declined. The frequency of CD8+ CD127+ T cells in chronic HBV infection was lower than the control group and self-limited infection group,especially in immune tolerance with HBeAg+ and immune clearance phase. Conclusion: The frequencies of antigen specific CD8+ T cells are not the main determinant of immune-mediated protection in chronic HBV infection,memory antigen specific CD8+ T cells are not clear or missing,which provides the possibility for therapeutic vaccines and immunization therapy.关键词
乙肝病毒/感染不同阶段/T淋巴细胞/免疫功能Key words
Hepatitis B virus/Different stages of infection/T lymphocytes/Immune function分类
医药卫生引用本文复制引用
杨新星,李东升,周继光,杨东亮,郝友华..乙肝病毒感染不同阶段抗原肽特异性细胞毒性 T 淋巴细胞的初步研究[J].中国免疫学杂志,2016,32(10):1496-1502,7.基金项目
本文受“十一五”艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2008ZX10002-011)和国家自然基金项目(No.30400412)资助。 （2008ZX10002-011）
