中国免疫学杂志2016，Vol.32Issue(10)：1503-1506,4.DOI:10.3969/j.issn.1000-484X.2016.10.020
单核巨噬细胞极化在慢性活动性乙型肝炎中的表达及意义
Expression and significance of monocyte-macrophage polarization in chronic active hepatitis B patients
摘要
Abstract
Objective:To research the number and function of monocyte-macrophages in patients with chronic active hepatitis B. Methods:The 51 chronic viral hepatitis B( CHB) patients were selected randomly,which consisted of 20 cases of mild-moderate,31 cases of severe group and 13 cases of healthy controls. PBMCs were separated by percoll. Monocytes were tagged by CD14,the molecules CD80,CD86,HLA-DR and CD163 were detected by flow cytometry which expressed on the surface of PBMCs. Serum cytokine were detected for IL-10, IL-12 and IL-23 by ELISA. The distribution of CD68 was detected in the liver by immunohistochemical staining. Results:The expressions of CD80 for all chronic hepatitis B patients were lower than the controls respectively,no matter mild-moderate or even severe group. Similarly,the HBV patients expressed lower level of CD86 in the peripheral blood mononuclear cells when compared with the control group. Furthermore, there was statistically difference between the levels of CD86 in severe group compared with control group (P<0. 01). As the expression of CD80 and CD86,the levels of HLA-DR in the patents had also declined when compared with controls. While the HLA-DR levels in both the mild-moderate HBV hepatitis groups were statistically significant higher than the severe group (P<0. 01). Different from the above all,the expression of CD163 in all chronic HBV hepatitis was higher than the control group. The CD68 positive cells in chronic HBV patients were observed and infiltrated increasingly in portal area and hepatic lobules (P<0. 05). There were statistically significant differences of IL-10 levels between the mild-moderate group,severe group and the control group,respectively (P<0. 01). Conclusion:Macrophages have participated in the pathological lesions of liver in CHB patients,among peripheral blood mononuclear cells,the phenomena of imbalance between type M1/M2 and polarization to type M2 have been observed,which participated in the development of the chronicity of CHB.关键词
单核细胞/巨噬细胞/极化/乙型肝炎Key words
Mononuclear cell/Macrophages cell/Polarization/Viral hepatitis B分类
医药卫生引用本文复制引用
杨江华,林敏,孙静,梁曼曼,王文节..单核巨噬细胞极化在慢性活动性乙型肝炎中的表达及意义[J].中国免疫学杂志,2016,32(10):1503-1506,4.基金项目
本文为国家自然科学基金主任基金(81141083)和安徽省高校省级自然科学研究项目(KJ2014A271)。 （81141083）
