中华骨质疏松和骨矿盐疾病杂志2016，Vol.9Issue(3)：289-295,7.DOI:10.3969/j.issn.1674-2591.2016.03.012
铁蓄积骨质疏松小鼠模型造血干细胞与骨代谢指标的相关性
Correlation between hematopoietic stem cells and bone turnover markers in iron accumulation osteoporosis mouse model
摘要
Abstract
Objective To explore the correlation between hematopoietic stem cells (HSCs) and bone turnover markers in iron accumulation osteoporosis mice model .Preliminary discussing the role of iron accumulation osteoporosis in hematopoieticsystem.Methods 6-8weeksC57malemicewereintraperitoneallyinjectedwith0.1g/kgferricammoni-um citrate ( FAC ) for eight weeks, serum ferritin, osteogenesis marker procollagen type 1 N-terminal propeptide (P1NP), osteoclastic markers CTX, peripheralroutine blood were detected , using micro-CT for distal femur trabecular bone reconstruction of three dimensional form and spatial structure parameter analysis , HSCs accounts for the proportion of bone marrow cells were detected by flow cytometry , HSCs cell colony forming ability was measured . Results The ELISA results show that the FAC intervention group increased bone marrow HSCs proportion 4.01%±0.63% compared to control group 2.61%±0.43% ( P<0.05 ) , and HSCs cell colony formation 142/dish decreased compared to control group 47/dish; FAC group increased serum ferritin ( 13.27 ±0.72 ) μg/L compared to control group ( 4.14 ± 0.96) μg/L, decreased P1NP ( 4.62 ±0.76 ) ng/ml compared to control group ( 8.23 ±0.66 ) ng/ml, CTX (123.54 ±1.63) ng/ml increased compared to control group (83.36 ±1.89) ng/ml (P <0.05); FAC group in-creased peripheral blood platelet (1380 ±103) ×109/L compared to control group (1023 ±83) ×109/L (P<0.05);Micro-CT results showed FAC group lower BMD ( 50.41 ±10.27 ) mg/mm3 compared to control group ( 104.68 ± 8.56 ) mg/mm3 , bone trabecular space structure parameters decreased obviously [ BV/TV 10.18%±1.76%; Tb.Th (0.057 ±0.012) mm;Tb.N (1.02 ±0.21) N/mm] compared to control group [BV/TV 19.92%±1.92%; Tb.Th (0.098 ±0.008) mm; Tb.N (1.45 ±0.13) N/mm] (P<0.05).Conclusion Iron accumulation has significant effects on both bone turnover markers and HSCs .关键词
铁蓄积/骨质疏松/造血干细胞Key words
iron accumulation/osteoporosis/hematopoietic stem cells分类
医药卫生引用本文复制引用
袁晔,徐又佳,王亮,曹彦,徐莉,徐非,俞晨,沈光思,杨帆,易剑桥..铁蓄积骨质疏松小鼠模型造血干细胞与骨代谢指标的相关性[J].中华骨质疏松和骨矿盐疾病杂志,2016,9(3):289-295,7.基金项目
国家自然科学基金（81273090，81572179，81302438，81500680，81502812）；苏州市卫生局项目（LCX201305）；苏州大学附属第二医院优势学科群（ XKQ2015001）；苏州大学附属第二医院预研项目 （）
