作物学报2016，Vol.42Issue(10)：1419-1428,10.DOI:10.3724/SP.J.1006.2016.01419
大豆14-3-3蛋白与转录因子蛋白GmMYB173的互作
Interaction of Soybean 14-3-3 Proteins with Transcription Factor GmMYB173
摘要
Abstract
14-3-3 proteins, nearly existing in all eukaryotic cells, may regulate many physiological and biochemical processes through interacting with other proteins. As the largest class of transcription factors in plants, MYB gene family is widely involved in plant growth and metabolism regulation. A gene cloned from soybean cultivar Zigongdongdou, and specifically expressed in the nuclear in the subcellular location assay. Sequence analysis showed that there was a binding site of 14-4-4 proteins, namely the pST binding site, in GmMYB173 similar to that in GmMYB176. The nuclear-specific expression of GmMYB173 disappeared when the sequence of the pST binding site was deleted through the splicing by overlap extension PCR. All 14-3-3 proteins from GmSGF14a to GmSGF14p could interact with GmMYB173. Among them, GmSGF14n interacted with GmMYB173 strongest, GmSGF14e and GmSGF14k took second place, which was proved by theβ-galactosidase activity analysis. These results suggest that 14-3-3 proteins not only interact with GmMYB173, but also probably regulate its subcellular location. The information pro-vided by this study will facilitate the study of interaction relationship between 14-3-3 proteins and GmMYB173 and its function on the soybean development.关键词
14-3-3蛋白/GmMYB173/亚细胞定位/酵母双杂交Key words
14-3-3 proteins/GmMYB173/Subcellular location/Yeast two-hybrid引用本文复制引用
董萌,高友菲,韩天富,东方阳,蒋炳军..大豆14-3-3蛋白与转录因子蛋白GmMYB173的互作[J].作物学报,2016,42(10):1419-1428,10.基金项目
本研究由国家现代农业产业技术体系建设专项(CARS-04)和中国农业科学院科技创新工程项目资助。This study was supported by the Special Program of Modern Agro-industry Technology System (CARS-04) and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences （CARS-04）
