作物学报2016，Vol.42Issue(10)：1429-1436,8.DOI:10.3724/SP.J.1006.2016.01429
抗根腐病的TaMYB86过表达转基因小麦的创制与分子功能鉴定
Development and Characterization ofTaMYB86-Overexpressing Transgenic Wheat Lines with Resistance to Common Root Rot
摘要
Abstract
Wheat common root rot, mainly caused byBipolaris sorokiniana, is a difficultly prevent soil-borne disease of wheat (Triticum aestivum L.) worldwide.TaMYB86, a B. sorokiniana-induced wheat MYB gene,encodes a MYB transcription factor. We constructed theTaMYB86 overexpression vector pUbi:MYC-TaMYB86 and introducedTaMYB86 into Yangmai 16 via the particle bombardment. TheTaMYB86 transgenic wheat lines on generations of T0–T3 were underwent by molecular characteristics analysis and disease resistance evaluation. The PCR and quantitative RT-PCR results showed that the alienTaMYB86was intro-duced into three transgenic wheat lines, and the relative transcriptional level ofTaMYB86was apparently higher in transgenic wheat lines than in non-transformed Yangmai 16. As Western blot results presented, the introducedMYC-TaMYB86 gene was translated into the MYC-TaMYB86 protein in the three overexpressing transgenic lines, but not in non-transformed Yangmai 16. The infection types and disease indexes of threeTaMYB86 transgenic wheat lines were significantly lower than those of non-transformation Yangmai 16 (t-test,P< 0.01). The transcript levels of 3 wheat defense genes (PR10, PR17c,and Chit1) were significantly elevated in three transgenic wheat lines than in the non-transformed Yangmai 16. These results indicate that overex-pression ofTaMYB86 enhances significantly resistance toB. sorokiniana in transgenic wheat lines and TaMYB86 plays a positive role in defense response toB. sorokiniana.关键词
小麦/MYB转录因子/防御反应/小麦根腐病/转基因Key words
Triticum aestivum/MYB transcription factor/Defense response/Common root rot/Transgene引用本文复制引用
单天雷,洪彦涛,杜丽璞,徐惠君,魏学宁,张增艳..抗根腐病的TaMYB86过表达转基因小麦的创制与分子功能鉴定[J].作物学报,2016,42(10):1429-1436,8.基金项目
本研究由国家转基因生物新品种培育重大专项(2016ZX08002-001-004)和国家自然科学基金项目(31471494)资助。The study was supported by the National Major Project for Developing New GM Crops (2016ZX08002-001-004) and the National Natural Science Foundation of China (31471494) （2016ZX08002-001-004）
