作物学报2016，Vol.42Issue(10)：1462-1470,9.DOI:10.3724/SP.J.1006.2016.01462
玉米光敏色素A1与A2在各种光处理下的转录表达特性
Transcription Characteristics ofZmPHYA1 and ZmPHYA2 under Different Light Treatments in Maize
摘要
Abstract
Plant phytochromes are a family of red/far-red light photoreceptors, which have two forms in plant: inactive red light ab-sorbing form (Pr) and active far-red light absorbing form (Pfr). During plant growth and developmental processes, phytochromes play pivotal roles in regulations of seed germination, plant height, flowering time, and shade-avoidance. In the grasses, three subfamilies are present:PHYA,PHYB andPHYC. In maize, an ancient genome duplication has increased the family member to six:PHYA1, PHYA2,PHYB1,PHYB2,PHYC1, andPHYC2. Phytochrome A facilitates the inhibition of hypocotyl elongation, opening of the api-cal hook, expansion of cotyledons, accumulation of anthocyanin and blocking of greening by continuous FR (FRc) light. In order to evaluate the light response capability and difference of transcription abundance betweenZmPHYA1 andZmPHYA2, we employed quantitative real-time PCR (qRT-PCR) assay to investigate the expression patterns ofZmPHYA1 andZmPHYA2 in the inbred line B73 and Mo17 with different light treatments. The results indicated that both ZmPHYA1 andZmPHYA2 had a high expression level in leaf and silk, and the transcription abundance ofZmPHYA1 was 2–8 times higher than that ofZmPHYA2. Inbred lines of both B73 and Mo17 possessed longer mesocotyls in dark, far-red and blue light conditions than in red or white light conditions. BothZmPHYA1 and ZmPHYA2 had a high expression level in far-red and blue lights and rapidly responded to dark-to-far-red and dark-to-blue transitions. ZmPHYA1 was more important under far-red light, so wasZmPHYA2 in blue light. Both of the genes could rapidly respond to transi-tions from dark to red or white light with similar expression pattern. The both genes also respond to long-day or short-day treatments, however the transcription abundance ofZmPHYA1was 2–5 times higher than that ofZmPHYA2during the treatments. All the results suggested that the transcription of bothZmPHYA1 andZmPHYA2 could rapidly responded to different light treatments;ZmPHYA1 might be more effective thanZmPHYA2 in crop improvement. Our results provide a theoretical basis for the function study and evaluation of light response ability for bothZmPHYA1 andZmPHYA2.关键词
玉米/光敏色素/光信号转导/表达分析/光处理Key words
Maize/Phytochrome/Light signaling transduction/Expression analysis/Light treatment引用本文复制引用
杨宗举,闫蕾,宋梅芳,苏亮,孟凡华,李红丹,白建荣,郭林,杨建平..玉米光敏色素A1与A2在各种光处理下的转录表达特性[J].作物学报,2016,42(10):1462-1470,9.基金项目
本研究由国家重点研发计划试点专项(SQ2016ZY03002918),国家转基因生物新品种培育重大专项(2016ZX08010002-003-002),北京市自然科学基金(重点)项目(6151002)和中国农业科学院科技创新工程项目资助。This study was supported by the National Research and Development Program (SQ2016ZY03002918), the Genetically Modified Organisms Breeding Major Projects of China (2016ZX08010002-003-002), the Key Project of Beijing Natural Science Foundation (6151002) and the Agricultural Science and Technology Innovation Program of CAAS （SQ2016ZY03002918）
