作物学报2016，Vol.42Issue(10)：1471-1478,8.DOI:10.3724/SP.J.1006.2016.01471
甘蓝型油菜BnFAD2-C5基因启动子及内含子在表达水平的功能分析
Functional Analysis ofBnFAD2-C5 Promoter and Intron at Expression Level in Brassica napus
摘要
Abstract
High oleic rapeseed breeding and the formation mechanism of oleic acid have become a central issue after finding the important economic value of rapeseed oil with high oleic acid. The fatty acid dehydrogenase gene (FAD2) is a key enzyme gene to control oleic acid content, but the regulation ofFAD2 gene is not well understood. According to the homology between rapeseed and oleracea, theBnFAD2-C5 promoter sequence of 1257 bp was cloned. Promoter and intron ofBnFAD2-C5gene were analyzed usingβ-glucuronidase (GUS) reporter and green fluorescent protein (GFP) reporter system to construct deleted vectors and trans-formArabidopsis thaliana.Deletion analysis ofBnFAD2-C5 promoter through GUS stainning revealed that –319 to –1 bp was the minimum promoter region. And deletion analysis ofBnFAD2-C5 promoter and intron through GFP reporter system using western technique showed that –1257 to –1020 bp and –319 to –1 bp regionsofBnFAD2-C5 promoter could induce expression of reporter genes effectively in transgenicArabidopsisseed in the mid stage of seed development, whileBnFAD2-C5intron could confer the enhancement of promoter’s function and the intron-mediated enhancement region was mainly located in +631 to +1033 bp.关键词
甘蓝型油菜/BnFAD2-C5基因/内含子增强效应/拟南芥Key words
Brassica napus/BnFAD2-C5gene/Intron-mediated enhancement/Arabidopsis thalina引用本文复制引用
刘睿洋,刘芳,张振乾,官春云..甘蓝型油菜BnFAD2-C5基因启动子及内含子在表达水平的功能分析[J].作物学报,2016,42(10):1471-1478,8.基金项目
本研究由湖南省科技创新项目(CX2013A012)和国家重点基础研究发展规划项目(2015CB150200)资助。This study was supported by the Science and Technology Innovation Project of Hunan Province (CX2013A012) and the National Basic Re-search Program of China (2015CB150200) （CX2013A012）
