作物学报2016，Vol.42Issue(10)：1487-1494,8.DOI:10.3724/SP.J.1006.2016.01487
玉米氮素敏感性差异自交系的表达谱分析
Global Transcriptome Analysis in High- and Low-Nitrogen Responsive Inbred Lines of Maize
摘要
Abstract
Different maize genotypes exhibit variation in nitrogen (N) responsiveness, however, the corresponding gene expres-sion patterns are unexplored yet. Here, we performed a comprehensive transcriptome profiling of high- and low-nitrogen respon-siveness genotypes in the conditions of sufficient-nitrogen (SN) and limiting-nitrogen (LN) supply. Under LN condition, B73, a high-N-responsive inbred line, accumulated a much lower N content in leaf than under SN condition. Whereas, the Mo17, a low-N-responsive inbred line, showed no significant difference in leaf N content between SN and LN. The RNAseq assay re-vealed a total of 13 867 and 10 028 differentially expressed genes in Mo17 and B73, respectively, including 9044 (Mo17) and 4233 (B73) up-regulated genes and 4823 (Mo17) and 5795 (B73) down-regulated under LN. Moreover, the principal component analysis (PCA) showed that the magnitude of transcriptome variation was greater in Mo17 than in B73 under LN. According to two-tail variance analysis, 342 differentially expressed genes had significant genotype-by-nitrogen interaction. These genes were mainly involved in amino acid metabolism, photosynthesis, biosynthesis of secondary metabolites, and gene replication and ex-pression. Our results suggest that more genes might be activated under N limiting condition in the low-N-responsive genotype than in the high-N-responsive genotype in order to modulate N assimilation and utilization in maize plant.关键词
玉米/氮素/氮胁迫/转录组分析/氮素利用率Key words
Maize/Nitrogen/Nitrogen stress/Transcriptome profiling/Nitrogen use efficiency (NUE)引用本文复制引用
葛敏,吕远大,张体付,周玲,林峰,赵涵..玉米氮素敏感性差异自交系的表达谱分析[J].作物学报,2016,42(10):1487-1494,8.基金项目
本研究由国家自然科学基金项目(31271728)和江苏省农业科技自主创新资金项目(CX(14)2009)资助。This work was supported by the Natural Science Foundation of China (31271728) and the Jiangsu Agriculture Science and Technology Inno-vation Fund [CX(14)2009]。 （31271728）
