作物学报2016，Vol.42Issue(10)：1541-1550,10.DOI:10.3724/SP.J.1006.2016.01541
低温胁迫下白菜型冬油菜差异蛋白质组学及光合特性分析
Differential Proteomic Analysis and Photosynthetic Characteristics of Winter Rapeseed under Low Temperature Stress
摘要
Abstract
Isolation and identification of the differentially expressed proteins in winter rapeseed at low temperature, laid a founda-tion for revealing the mechanism of cold resistance of winter rapa. The strong cold resistant winter rape Longyou 7 was used as experimental material. Two dimensional electrophoresis, mass spectrometry and retrieval techniques were used to compare the proteomic differences at low temperature (4℃, –4℃) and normal atmospheric temperature (25℃/20℃), and functions were analyzed by KO and KEGG. It was observed that Longyou 7 had subsided growth point, creeping stem, and closed or semi-closed stomata under low temperature. The 2-DE and PDQuest8.0.1 software analysis showed that the number of protein spots were 726 and 738, respectively. Compared with the normal temperature treatment, at the 4℃ treatment showed differential expression at 10 protein spots while didn’t at five protein. Eleven proteins were identified by MS MALDI-TOF-TOF mass spectrometry analysis. They are involved in carbohydrate metabolism, rugan metabolism, amino acid metabolism, organic acid metabolism, nucleic acid metabolism, signal transduction in cell and communication and other cellular processes. we found the high activity of antifreeze protein in Longyou 7 leaf protein extraction solution after low temperature treatment through ice crystal morphology microscopic observation. Five out of the 11 proteins identified were associated with photosynthesis. Under low temperature, ribulose 1,5-bisphosphate carboxylase (RuBPCase) activity and photosynthetic rate decreases in leaves of Longyou 7. Under the low tem-perature, the proteome of winter rape was significantly changed, and the specific protein was expressed. The decrease ofPn in leaves was related to the expression inhibition and the activity decrease of RuBPCase. The decrease ofPn in leaves was mainly caused by non stomatal limitation. High activity of antifreeze protein plays an important role in cold resistance of winter rape.关键词
白菜型油菜/低温胁迫/蛋白质组学/光合特性Key words
Winter rape/Low temperature stress/Protein proteomics/Photosynthetic characteristics引用本文复制引用
刘自刚,米超,袁金海,孙万仓,曾秀存,方彦,王志江,武军艳,方园,李学才..低温胁迫下白菜型冬油菜差异蛋白质组学及光合特性分析[J].作物学报,2016,42(10):1541-1550,10.基金项目
本研究由国家自然科学基金项目(31460356),国家农业科技成果转化资金项目(2014G10000317),国家现代农业产业技术体系建设专项(CARS-13)和国家重点基础研究发展计划项目(2015CB150206)资助。This study was supported by the National Science Foundation of China (31460356), the National Agricultural Science and Technology Achievements Transformation Fund of China (2014G10000317), the China Agriculture Research System (CARS-13), and the National Basic Research Program of China (973 Program)(2015CB150206) （31460356）
