北京林业大学学报2016，Vol.38Issue(9)：16-24,9.DOI:10.13332/j.1000--1522.20150423
基于SRAP分子标记的刨花润楠遗传多样性分析
Genetic diversity of Machilus pauhoi assessed by SRAP markers
摘要
Abstract
The sequence-related amplified polymorphism ( SRAP ) markers were used to assess the genetic diversity and relationship of 23 Machilus pauhoi Kanehira provenances from seven provinces. Twelve primers were screened out which were used for SRAP-PCR. The results showed that:1 ) a total of 157 clear bands were amplified, and 115 bands were polymorphic ( the percentage of polymorphic band, PPB=73. 24%);2) the polymorphism information content (PIC) values ranged from 0. 406 to 0. 502, with an average of 0. 476;3) the molecular variance analysis ( AMOVA) revealed that genetic variation mainly existed within provenance ( 72. 19%) and few among provenances ( 27. 81%) , showing that the variation of M. pauhoi within provenances was the major source of genetic diversity; 4 ) the cluster analysis results suggest that 23 provenances should be divided into four categories: provenances from Hunan, Guangdong, Guangxi and Jiangxi are clustered in the same group;all provenances from Zhejiang Province are together; Wuyuan and Huangshan provenances are classed together; and all provenances from Fujian Province are clustered into one group, The classification results reflect geographic trends significantly. The result of this study provides theoretical basis for germplasm resources preservation and breeding programs of M. pauhoi.关键词
刨花润楠/相关序列扩增多态性(SRAP)/遗传多样性Key words
Machilus pauhoi Kanehira/sequence-related amplified polymorphism/genetic diversity分类
农业科技引用本文复制引用
周鹏,林玮,朱芹,周祥斌,吴林瑛,陈晓阳..基于SRAP分子标记的刨花润楠遗传多样性分析[J].北京林业大学学报,2016,38(9):16-24,9.基金项目
“十二五”国家科技支撑计划项目(2012BAD01B04)、广东省林业科技创新项目(2011KJCX002)。 （2012BAD01B04）
