北京大学学报（医学版）2016，Vol.48Issue(5)：777-782,6.DOI:10.3969/j.issn.1671-167X.2016.05.005
尼古丁减轻高脂高果糖诱导的非酒精性脂肪性肝炎小鼠的肝脏炎症
Nicotine alleviates the liver inflammation of non-alcoholic steatohepatitis induced by high-fat and high-fructose in mice
摘要
Abstract
Objective:To investigate the anti-inflammation effects by activation of the cholinergic anti-inflammatory pathway and its mechanisms in non-alcoholic steatohepatitis (NASH)model mice.Me-thods:6-week-old male C57BL/6J (B6)mice were randomly divided into four groups:the first group was normal mice,injected with saline;the second group was normal mice,injected with nicotine;the third group was NASH model mice,injected with saline;the fourth group was NASH model mice,injec-ted with nicotine.The experimental mice were fed with either standard chow (SC)or high-fat and high-fructose (HFHF)for 17 weeks to generate an NASH model mice.The mice received injection once daily for 3 weeks [nicotine dose,400 μg/kg].Then,their pathological characteristics and function of the liver were assessed.The expressions of interleukin-6 (IL-6)and tumor necrosis factor-α(TNF-α)in se-rum were analyzed by enzyme linked immunosorbent assay (ELISA).The expressions of alpha 7 nicotinic acetylcholine receptors (α7nAChR),Toll-like receptors-4 (TLR-4)and nuclear factor κB of phosphory-lation (p-NF-κB)in Kupffer cells were determined by Western blot and immunofluorescence assays.Re-sults:We successfully generated NASH model mice by imitating the high-fat and high-fructose dietary style of NASH patients.The results of our investigation demonstrated that nicotine could reduce signifi-cantly the levels of IL-6,and TNF-αin serum (P <0.05).The expression of p-NF-κB protein in the group which was NASH model mice injected with nicotine declined significantly as compared with the group which was NASH model mice injected with saline (P <0.05).And the expression of α7nAChR protein elevated significantly conversely (P <0.05 ).Conclusion:Activation of the cholinergic anti-inflammatory pathway could inhibit the release of inflammatory factors as TNF-αand IL-6 in NASH model mice,and the mechanism for the inhibition of inflammatory was mediated by NF-κB pathway.关键词
尼古丁/非酒精性脂肪性肝病/受体,胆碱能/炎症介导素类/小鼠Key words
Nicotine/Non-alcoholic fatty liver disease/Receptors,cholinergic/Inflammation media-tors/Mice分类
医药卫生引用本文复制引用
陈小梅,李富强,严速,吴小翠,唐翠兰..尼古丁减轻高脂高果糖诱导的非酒精性脂肪性肝炎小鼠的肝脏炎症[J].北京大学学报（医学版）,2016,48(5):777-782,6.基金项目
国家自然科学基金（81100279）和浙江省新苗人才计划项目（2014R410058）资助 Supported by the National Natural Science Foun-dation of China （81100279）and Xin-miao Talent Program of Zhejiang Province （）
