北京大学学报（医学版）2016，Vol.48Issue(5)：860-865,6.DOI:10.3969/j.issn.1671-167X.2016.05.021
转化生长因子-β1和血管内皮生长因子在浓缩生长因子各层中的分布及含量特点
Distribution and content of transforming growth factor-β1 and vascular endothelial growth factor in each layer of concentrated growth factors
摘要
Abstract
Objective:To investigate the distribution and content of transforming growth factor-β1 (TGF-β1)and vascular endothelial growth factor (VEGF)in concentrated growth factors (CGF)gel, and to clarify the difference among different layers of CGF.Methods:Venous blood samples were col-lected from 6 healthy volunteers to prepare CGF.The distribution,integrated optical density (IOD)and average optical density (AOD)of TGF-β1 and VEGF in CGF gel and red blood cell (RBC)layer were measured using immunohistochemistry.The concentrations of TGF-β1 and VEGF in the supernatant se-rum at baseline and the CGF releasate after 1 day were evaluated with enzyme-linked immunosorbent as-says.Results:Abundant TGF-β1 and VEGF were concentrated in CGF gel.However,only a little could be found in polykaryocytes and sporadic platelets in RBC layer.Platelets and leukocytes were concentra-ted in between the two layers with high expression of TGF-β1.The concentrations of TGF-β1 and VEGF in the CGF releasate(55 236.78 ±3 686.34),(610.99 ±148.81)ng/L were significantly higher than those in the supernatant serum (20 710.20 ±4 523.14),(335.20 ±51.69 )ng/L (P <0.001 ). Conclusion:CGF contains high quantities of TGF-β1 that can promote new bone formation and tissue healing.We suggest that CGF gel should be used right after being prepared.Supernatant serum and the area between CGF gel and RBC layer could also be mixed with bone substitute materials.关键词
浓缩生长因子/转化生长因子 β1/血管内皮生长因子/骨生成Key words
Concentrated growth factors/Transforming growth factor beta1/Vascular endothelial growth factors/Osteogenesis分类
医药卫生引用本文复制引用
陈飞,潘韶霞,冯海兰..转化生长因子-β1和血管内皮生长因子在浓缩生长因子各层中的分布及含量特点[J].北京大学学报（医学版）,2016,48(5):860-865,6.基金项目
国家临床重点专科建设项目（2011）和教育部留学回国人员科研启动基金（2012-940）资助 Supported by the National Key Clinical Specialist Construction Programs of China （2011）and the Scientific Research Foundation for the Returned Overseas Chinese Scholars，Ministry of Educa-tion of China （）
