草业科学2016，Vol.33Issue(9)：1658-1664,7.DOI:10.11829/j.issn.1001-0629.2015-0617
一株野大麦内生真菌的生物学与生理学特性
Biological and physiological characteristics of Epichloëbromicola endophyte symbiotic with Hordeum brevisubulatum
摘要
Abstract
Biological and physiological characteristics of endophyte Epichloëbromicola strain WBE1 isolated from wild barley seeds were measured in a range of temperatures,pH,light,carbon source,nitrogen source and growth rate values.WBE1 was able to grow at temperate from 10 ℃ to 30 ℃ with the optimal temperature of 22~25 ℃ and stop growing at 4 ℃ and 35 ℃.The optimal pH for mycelial growth ranged 5.09 to 6.1.The ability of carbon source utilization decreased in the following order:maltose > D-sorbose > mannose > su-crose > lactose > starch > glucose.However,D-xylose inhibited the mycelial growth.The ability of nitrogen source utilization decreased in the following order:casein tryptone > beef extract > peptone > yeast > L-me-thionine > L-phenylalanine > leucine.However,potassium nitrate,praline and ammonium oxalate cannot be utilized by WBE1 and ammonium molybdate inhibited mycelial growth.Potato dextrose agar (PDA),oat meal agar (OMA),corn meal agar (CMA)and potato sugar agar (PSA)were optimal medium for WBE1 .The my-celial growth rate on PDA reached maximum at the sixth week,then decreased gradually afterwards.关键词
野大麦/内生真菌/生物学特性/生理学特性Key words
wild barley/endophyte/biological characteristics/physiological characteristic分类
农业科技引用本文复制引用
陈泰祥,李春杰,李秀璋..一株野大麦内生真菌的生物学与生理学特性[J].草业科学,2016,33(9):1658-1664,7.基金项目
国家重点基础研究发展规划(“973”计划)(2014CB138702) （“973”计划）
国家自然科学基金(31372366) （31372366）
教育部创新团队发展计划(IRT13019) （IRT13019）
兰州大学中央高校基本业务费专项资金(lzujbky-2016-180) （lzujbky-2016-180）
