草业科学2016，Vol.33Issue(9)：1673-1680,8.DOI:10.11829/j.issn.1001-0629.2016-0304
不同浓度镉胁迫对孔雀草DNA甲基化的影响
Effects of different concentrations Cd stress on DNA methylation of Tagetes patula
摘要
Abstract
With the speeding up of human’s activity and industrialization process,heavy metal pollution has caused serious harm to the environment.In the present study,the methylation-sensitive amplified polymor-phism (MSAP)technique was employed to analyze the DNA methylation in Tagetes patula under different concentrations of cadmium (Cd)in pots experiment.The results showed that the genomic MSAP rate of T.patula was 24%,30%,35% and 41%,and the total methylation rate of T.patula was 20%,23%,25%and 27%,respectively,when treated with the different concetrations of Cd2+(0,50,250,500 mg•kg-1 ).The results indicated that the total methylation level of DNA increased with the increase of Cd2+ concentration.Mo-reover,in regards of the change pattern of DNA methylation,the denove methylation rates were 10%,10%and 11%,respectively,under stress of 50,250 and 500 mg•kg-1 Cd2+,which indicated that the denove meth-ylation was the main methylation pattern.In summay,the current study provides a theoretical reference for re-vealing the variation trend of heavy metal stress on plant DNA methylation and the mechanism of plant toler-ance to heavy metal stress.关键词
孔雀草/Cd2+胁迫/DNA甲基化/MSAP技术Key words
Tagetes patula/Cd stress/DNA methylation/MSAP technique分类
农业科技引用本文复制引用
张凯凯,陈兴银,杨鹏,关萍..不同浓度镉胁迫对孔雀草DNA甲基化的影响[J].草业科学,2016,33(9):1673-1680,8.基金项目
贵州大学研究生创新基金(研农2016005) （研农2016005）
贵州省社会发展攻关课题(201303137) （201303137）
