动物医学进展2016，Vol.37Issue(10)：11-15,5.
2014年-2015年天津地区 PRRSV 分子流行病学调查及流行毒株的分离鉴定
Isolation,Identification and Molecular Epidemiology of Porcine Reproductive and Respiratory Syndrome Virus in Tianjin from 2014 to 2015
孙英峰 1付永利 2于海霞 3张立新 3张超 3杨汉春 4李志 1路超 3韩伟 5任卫科 5李富强 5田向学 5张莉 5李秀丽5
作者信息
- 1. 中国农业大学动物医学院，北京 100094
- 2. 天津市畜牧兽医研究所，天津 300384
- 3. 天津市畜牧总站，天津 300402
- 4. 石家庄市兽用生物制品供应站，河北石家庄 050000
- 5. 天津市畜牧兽医研究所，天津 300384
- 折叠
摘要
Abstract
This study aimed at analyzing the genetic variation and the development trend of PRRSV in Tian-jin from 2014 to 2015.237clinical samples collected from 2014 to 2015 in different districts of Tianjin were tested by RT-PCR to amplify partial Nsp2 and isolate virus using positive samples.The result showed that 64 samples were positive,the positive rate was 27%,and acquired 33 PRRSV isolates were field;partial Nsp2 gene and phylogenetic analysis revealed that most of pandemic strains were the North American pro-totype,and 54 samples were highly homologous with the highly pathogenic PRRSV(HP-PRRSV),8 sam-ples were highly homologous with classic PRRSV,2 samples were highly homologous with North American pandemic strain NADC30.The study indicated that HP-PRRSV was the popular strain in Tianjin during 2014-2015,the NADC30-like PRRSV emerged make the category of PRRSV variant more diverse,pointing that it is important to pay more attention to monitor prevalent and genetic variation of PRRSV.关键词
猪繁殖与呼吸综合征病毒/分离/分子流行病学/遗传演化分析Key words
Porcine reproductive and respiratory syndrome virus/isolation/molecular epidemiology/phylo-genetic analysis分类
农业科技引用本文复制引用
孙英峰,付永利,于海霞,张立新,张超,杨汉春,李志,路超,韩伟,任卫科,李富强,田向学,张莉,李秀丽..2014年-2015年天津地区 PRRSV 分子流行病学调查及流行毒株的分离鉴定[J].动物医学进展,2016,37(10):11-15,5.
