动物医学进展2016，Vol.37Issue(10)：16-20,5.
马链球菌对树突状细胞 Toll 样受体和 MyD88及细胞因子 mRNA 表达的影响
Effects of Streptococcus equi on mRNA Expressions of TLR1,TLR2,TLR6,MyD88 and Cytokines in Dendritic Cells
摘要
Abstract
To study the effects of Streptococcus equi subsp equi (S.equi )on the mRNA expressions of TLR1,TLR2,TLR6,MyD88,IL-1,IL-6,IL-10,IL-12 and TNF-α in mouse bone marrow derived dendritic cells (BMDCs)cultured in vitro ,Naive DCs were cultured with GM-CSF and IL-4,SYBR Green Ⅰfluores-cence quantitative PCR was used to detect mRNA transcriptional levels of TLRs,MyD88 and cytokines af-ter infection with S.equi about 16,20,26 h.The results showed that at 16,20,26 h post-infections,the ex-pression levels of TLR1,TLR2,TLR6 and MyD88 in S.equi infected BMDCs were significantly or ex-tremely significantly higher than those of the negative control group (P <0.05 or P <0.01 ).At 16,26 h post-infections,the concentration of IL-1 had no obviously change (P >0.05),IL-10,TNF-α were signifi-cantly or extremely significantly increased than those of the negative control group (P <0.05 or P <0.01). IL-6 was significantly increased at 16 h post-infection (P <0.05),however,it had no change at 26 h post-infection (P > 0.05).At 16 h post-infection,the concentration of IL-12 had no obviously change (P >0.05),while at 26 h post-infection,it was significantly increased (P <0.05).These results indicated that S.equi was able to regulate the mRNA expressions of TLRs,MyD88 and cytokines,which mediated im-mune responses.关键词
马链球菌马亚种/树突状细胞/Toll样受体/细胞因子Key words
Streptococcus equi subsp.equi/dendritic cell/Toll-like receptor/cytokine分类
农业科技引用本文复制引用
杜润慈,冉多良,李静,付强,刘建华..马链球菌对树突状细胞 Toll 样受体和 MyD88及细胞因子 mRNA 表达的影响[J].动物医学进展,2016,37(10):16-20,5.基金项目
国家科技支撑计划项目 （）
