动物医学进展2016，Vol.37Issue(10)：60-63,4.
大熊猫犬瘟热病毒 LG 株的分离鉴定
Identification of Canine Distemper Virus LG Strain Isolated from Giant Panda
摘要
Abstract
To isolate a wild strain of canine distemper virus (CDV),after 3 times repeated grinding and freezing,the supernatants of tissue samples of giant panda including heart,lung and liver were inoculated in monolayer Vero cells,and harvested when viruses cause apperently cytopathic effect (CPE).The isolate was identified by reverse transcription polymerase chain reaction (RT-PCR)TCID50 of CDV was calculated by the Reed-Muench algorithm.The virulence of this isolate was assessed by animal regression assay.The result showed that the CPE such as shrink and pyknosis were observed in Vero cells after blindly passaging 5 times.The length of PCR amplification fragment was 287 bp,which coincided with the expected result. The nucleotide homology between the isolate and CDV SD (14)11 strain (Asia-Ⅰ)was 98%.The result of TCID50 was 10-5.2 TCID50/mL.The clinical symptoms of CD like body temperature increasing,nose ap-pearing dry,diarrhea,watery discharge secreted in eyes and nose and so on were observed after inoculation with this isolate.A CDV,named LG strain,was successfully obtained in this study from giant panda.关键词
犬瘟热病毒/大熊猫/分离鉴定Key words
CDV/giant panda/isolation and identification分类
农业科技引用本文复制引用
岳进华,刘鹤媛,周铭,高洋,杨启明,李雯丽,陈德坤..大熊猫犬瘟热病毒 LG 株的分离鉴定[J].动物医学进展,2016,37(10):60-63,4.基金项目
横向合作项目 （）
