南方医科大学学报2016，Vol.36Issue(10)：1328-1333,6.DOI:10.3969/j.issn.1673-4254.2016.10.05
猖獗龋患者的病因和口腔微生物种类分析：1例报告
Analysis of causes and whole microbial structure in a case of rampant caries
摘要
Abstract
Objective To analyze the whole microbial structure in a case of rampant caries to provide evidence for its prevention and treatment. Methods Clinical samples including blood, supragingival plaque, plaque in the caries cavity, saliva, and mucosal swabs were collected with the patient's consent. The blood sample was sent for routine immune test, and the others samples were stained using Gram method and cultured for identifying colonies and 16S rRNA sequencing. DNA was extracted from the samples and tested for the main cariogenic bacterium (Streptococcus mutans) with qPCR, and the whole microbial structure was analyzed using DGGE. Results The patient had a high levels of IgE and segmented neutrophils in his blood. Streptococci with extremely long chains were found in the saliva samples under microscope. Culture of the samples revealed the highest bacterial concentration in the saliva. The relative content of hemolytic bacterium was detected in the samples, the highest in the caries cavity;C. albicans was the highest in the dental plaque. In addition, 33 bacterial colonies were identified by VITEK system and 16S rDNA sequence phylogenetic analysis, and among them streptococci and Leptotrichia wade were enriched in the dental plaque sample, Streptococcus mutans, Fusobacterium nucleatum, and Streptococcus tigurinus in the caries cavity, and Lactobacillus in the saliva. S. mutans was significantly abundant in the mucosal swabs, saliva and plaque samples of the caries cavity as shown by qPCR. Compared to samples collected from a healthy individual and another two patients with rampant caries, the samples from this case showed a decreased bacterial diversity and increased bacterial abundance shown by PCR-DGGE profiling, and multiple Leptotrichia sp. were detected by gel sequencing. Conclusion The outgrowth of such pathogenic microorganisms as S. mutans and Leptotrichia sp., and dysbiosis of oral microbial community might contribute to the pathogenesis of rampant caries in this case.关键词
猖獗龋/口腔微生物/梅里埃生化鉴定/16S rDNA序列进化分析Key words
rampant caries/oral microbial flora/Vitek bacteria identification/16S rDNA sequence phylogenetic analysis引用本文复制引用
胡晓宇,李燕,姚羽菲,崔博淼,吕俊,沈鑫,任彪,李明云,郭强,黄睿洁..猖獗龋患者的病因和口腔微生物种类分析：1例报告[J].南方医科大学学报,2016,36(10):1328-1333,6.基金项目
国家自然科学基金（81172579）；四川科技厅科技支撑计划（2013SZ0039） Supported by National Natural Science Foundation of China (81172579) （81172579）
