南方医科大学学报2016，Vol.36Issue(10)：1340-1344,1356,6.DOI:10.3969/j.issn.1673-4254.2016.10.07
类风湿关节炎患者血清MBL、MASP-2、HsCRP与C3水平的相关性
Correlation among serum MBL, MASP-2, HsCRP and C3 levels in rheumatoid arthritis
摘要
Abstract
Objective To investigate the correlation among serum levels of manning-binding lectin (MBL), MBL-associated serine proteases-2 (MASP-2), complement C3 and high-sensitive C reactive protein (HsCRP) in patients with rheumatoid arthritis (RA). Methods Fasting venous blood were collected from 50 RA patients (25 in active stage and 25 in remission) and 40 healthy subjects for detecting serum levels of MBL, MASP- 2, complement C3 and HsCRP using enzyme- linked immunosorbent assay (ELISA) and immune turbidity assay. Results The serum levels of MBL and MASP-2 were significantly lower and HsCRP level was significantly higher in patients with RA (in both acute stage and remission) than in the healthy control group (P<0.05), but complement C3 level was similar between the RA patients and control group. Bivariate Pearson correlation analysis showed that in RA patients, MBL was positively correlated with MASP-2 level (r=0.550, P=0.001) and negatively with HsCRP (r=-0.323, P=0.022) but not correlated with C3 (r=-0.022, P=0.882); MASP-2 was negatively correlated with HsCRP (r=0.453, P=0.453) and was not correlated with C3 (r=0.049, P=0.738). ROC curve analysis revealed the largest area under curve (AUC) of HsCRP (0.844, P=0.001) and smaller AUCs of MBL (0.025, P=0.001) and MASP-2 (0.266, P=0.001). HsCRP had a much higher sensitivity (84%) than MBL (10%) and MASP-2 (40%) in the diagnosis of RA. Conclusion In RA patients, MBL and MASP-2 are negatively correlated with HsCRP level. Serum MBL and MASP-2 levels decrease with the progression of joint injury in RA patients, suggesting their involvement in the pathological process of RA;but due to their low sensitivity, they are not appropriate indicators for evaluating the disease activity of RA.关键词
类风湿关节炎/甘露糖结合凝集素/MBL相关的丝氨酸蛋白酶-2/补体系统/超敏C反应蛋白/补体C3Key words
rheumatoid arthritis/manning-binding lectin/MBL associated serine proteases-2/complement system/high sensi-tive C reactive protein引用本文复制引用
陈思,马宝良,曹明强,于红娟,马兴铭..类风湿关节炎患者血清MBL、MASP-2、HsCRP与C3水平的相关性[J].南方医科大学学报,2016,36(10):1340-1344,1356,6.基金项目
国家自然科学基金（31370912） Supported by National Natural Science Foundation of China (31370912) （31370912）
